Published on Chemistry (https://chemistry.iisuniv.ac.in)

Home > Chemistry Laboratory III

Chemistry Laboratory III [1]

Paper Code: 
25CCHY 213
Credits: 
02
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to

synthesize some organic compounds, purify them through crystallization and to inspect the purity through melting point and boiling point along with thin layer & paper chromatography.

 

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and Teaching Strategies

Assessment Strategies

Course Code

Course

title

 

25CCHY 213

 

 

Chemistry Laboratory III

(Practical)

 

CO33: Synthesize organic compounds and, purify them   using various solvents, including water, alcohol, and alcohol-water mixtures for crystallization.

CO34: Determine melting and boiling points of compounds using distillation and capillary methods and evaluate the effect of impurities.

CO35: Separate the components of a given mixture using paper and thin layer chromatography (TLC).

CO36: Compile and report the results efficiently, and develop communications skills.

CO37: Contribute effectively in course-specific interaction.

Approach in teaching:

Power point presentation, laboratory instructions and demonstrations.

 

 

Learning activities for the students:

Peer learning, hands-on-learning, interpretation of data, record

writing and learning through virtual labs.

 

Conduction of experiments, lab records, viva voce.

 

 

 

Unit I: 

Organic Synthesis

 

· Acetylation of aniline using green approach

· Bromination of acetanilide by conventional method/green approach

· Nitration of acetanilide by conventional method

· S-Benzylisothiouronium salt of of benzoic acid and oxalic acid.

 

 

Purification of organic compounds

· Crystallization of synthesized organic compounds using the following solvents: a. Water b. Alcohol c. Alcohol-Water      

· Determination of the melting points of above compounds and unknown organic compounds (electrically heated melting point apparatus)   

· Effect of impurities on the melting point: mixed melting point of two unknown organic compounds

· Determination of boiling point of liquid compounds. (boiling point lower than and more than 100 °C by distillation and capillary method)

 

Chromatography

Theory: Classification, principle and working of Thin Layer and Paper chromatography.

Experiments:

· Separation of a mixture of two amino acids by ascending and horizontal paper chromatography.

· Separation of a mixture of two sugars by ascending paper chromatography.

· Separation of a mixture of o-and p-nitrophenol or o-and p-aminophenol by thin layer chromatography (TLC) 

Essential Readings: 

1. Practical Organic Chemistry, Mann, F.G. & Saunders, B.C., Pearson Education, 2009

References: 

1. Advanced Practical Organic Chemistry, Jag Mohan, Himalaya Publishing House ,1992.

2. Vogel’s Textbook of Practical Organic Chemistry, Fifth Edition; B.S. Furniss, A.J. Hannaford, P.W.D. Smith, A.R. Tatchell; Pearson Education, New Delhi, 2003.

 

e-Resources:

 

1. https://www.slideshare.net/LavakusaBanavatu/thin-layer-chromatography-43293607 [2] (TLC)

2. https://www.studocu.com/in/u/23692705?sid=01684403081 (all [3] the experiments)

3. https://www.slideshare.net/msaltyy/paper-chromatography-65615443 [4] (Paper Chromatography)

Academic Year: 
2025-2026 [5]

Follow Chemistry on:

IIS(deemed to be University)

Gurukul Marg, SFS, Mansarovar, Jaipur 302020, (Raj.) India Phone:- +91-141-2400160-61, 2397906-07, Fax: 2395494, 2781158

Source URL: https://chemistry.iisuniv.ac.in/courses/subjects/chemistry-laboratory-iii-4

Links:
[1] https://chemistry.iisuniv.ac.in/courses/subjects/chemistry-laboratory-iii-4
[2] https://www.slideshare.net/LavakusaBanavatu/thin-layer-chromatography-43293607
[3] https://www.studocu.com/in/u/23692705?sid=01684403081%20(all
[4] https://www.slideshare.net/msaltyy/paper-chromatography-65615443
[5] https://chemistry.iisuniv.ac.in/academic-year/2025-2026