Analytical Biochemistry

Paper Code: 
BCH 202
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
9.00
Unit I: 
Chromatography-I

General principles and applications of:

  1. Adsorption Chromatography
  2. Ion-exchange Chromatography
  3. Thin-layer Chromatography
  4. Molecular-sieve Chromatography
  5. Hydrophobic Chromatography
     
9.00
Unit II: 
Chromatography-II

General principles and applications of:

  1. Gas-liquid chromatography
  2. HPLC
  3. Affinity chromatography
  4. Paper chromatography
     
9.00
Unit III: 
Electrophoretic Techniques

General principles. Factors affecting the migration rate- simple, electric field, buffer and supporting medium. Basic principles of agarose electrophoresis, PAGE and SDS-PAGE, Two-dimensional electrophoresis, its importance. Isoelectrofocussing.

9.00
Unit IV: 
Immunological Techniques

Immunoassays for infectious disease- Innate immunity, antigen-specific immunity and antibodies, cell-mediated immunity and Immune memory, antibody titers, skin tests and the diagnosis of infectious disease.
Radioimmunoassay and immunoblot procedures, immunodiffusion,  immunoelectrophoresis, ELISA, immunofluorescence.
 

9.00
Unit V: 
Radioisotopic Techniques

Types of radioactive decay-half life. Types of radioisotopes used in Biochemistry, units of radioactivity measurements, techniques used to measure radioactivity (gas ionization and liquid scintillation counting), nuclear emulsions used in biological studies (pre-mounted, liquid and stripping), isotopes commonly used in biochemical studies - 32P, 35S, 14C and 3H), Autoradiography. Biological hazards of radiation and safety measures in handling radioisotopes.
 

References: 
  1. Outlines of Biochemistry, Fifth edition, Eric E. Conn, Paul K. Stumpf, George Bruening, Roy H.   Doi, John Wiley & Sons, New Delhi, 2007.
  2. Physical Biochemistry, Second edition, David Friefelder, W.H. Freeman and Co., USA, 1983.
  3. Vogel’s Textbook of Quantitative Chemical Analysis, Fifth Edition, G.H. Jeffery, J. Bassett. J. Mendham, R.C. Denney, ELBS, England, 1991.
  4. Vogel’s Text Book of Practical Organic Chemistry, Fifth Edition, B.S. Furniss, A.J. Hannaford, P.W.G. Smith, A.R. Tatchell; Adission – Wesley Longman Ltd. England, 1998.
  5. An Introduction to Practical Biochemistry, Third edition, Plummer, Tata Mc Graw Hill, 2002.
  6. Brock Biology of Microorganisms, International edition, Ninth edition, Michael T. Madigan, John M. Martinko, Pearson Prentice Hall, 2000.
     
Academic Year: 
2018-2019
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