Biomolecules

Paper Code: 
24DCHY515(A)
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to

  • understand the fundamentals related to the electrochemical process and their applications.
  • gain an in-depth knowledge of theories of chemical kinetics and mechanism of catalyzed reactions.

 

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and Teaching Strategies

Assessment Strategies

Course Code

Course

Title

 

24DCHY515(A)

Biomolecules

(Theory)

 

 

 

CO112: Describe structural configuration, their synthesis & reactions of nucleic acids.

CO113: Discuss the theories of enzyme kinetics, the mechanisms of enzyme catalysis, and the mechanisms of enzyme regulation in the cell.

CO114: Explain the chemistry of lipids.

CO115: Comprehend the concept of energy in biosystems.

CO116: Learn about a variety of drug classes and their pharmacological actions.

CO117: Contribute effectively in course-specific interaction.

Approach in teaching:

Interactive lectures, tutorials, group discussions and e-learning.

 

 

Learning activities for the students:

Peer learning, e-learning, problem solving through tutorials and group discussions.

 

Written examinations, assignments and quiz.

 

 

12.00
Unit I: 
Nucleic Acids and Proteins

Components of nucleic acids, nucleosides and nucleotides, structure, synthesis and reactions of: adenine, guanine, cytosine, uracil and thymine, structure of polynucleotides.

α-Amino Acids: Synthesis, ionic properties and reactions, zwitterions, pKa values, isoelectric point and electrophoresis.

Study of peptides: Determination of their primary structures-end group analysis, methods of peptide synthesis: synthesis of peptides using N-protecting, C-protecting and C-activating groups: solid-phase synthesis.

Proteins: Classification and structure.

 

12.00
Unit II: 
Enzymes

Introduction, classification and characteristics of enzymes, salient features of active site of enzymes,

mechanism of enzyme action (taking trypsin as example), factors affecting enzyme action, coenzymes and cofactors and their role in biological reactions, specificity of enzyme action (including stereospecificity), enzyme inhibitors and their importance, phenomenon of inhibition (competitive, uncompetitive and non-competitive inhibition including allosteric inhibition).

12.00
Unit III: 
Lipids

Introduction to oils and fats, common fatty acids present in oils and fats, hydrogenation of fats and oils, saponification value, acid value, iodine number, reversion and rancidity.

 

12.00
Unit IV: 
Concept of Energy in Biosystems

Cells obtain energy by the oxidation of foodstuff (organic) molecules, introduction to metabolism (catabolism, anabolism).

ATP: The universal currency of cellular energy, ATP hydrolysis and free energy change, agents for transfer of electrons in biological redox systems: NAD+, FAD.

Conversion of food to energy: Outline of catabolic pathways of carbohydrate: glycolysis, fermentation, Krebs cycle.

Overview of catabolic pathways of fat and protein.

Interrelationship in the metabolic pathways of protein, fat and carbohydrate.

Caloric value of food, standard caloric content of food types.

12.00
Unit V: 
Pharmaceutical Compounds

Structure and importance, classification, structure and therapeutic uses of antipyretics: Paracetamol (with synthesis), analgesics: Ibuprofen (with synthesis), antimalarials: Chloroquine (with synthesis), an elementary treatment of antibiotics and detailed study of chloramphenicol, medicinal values of curcumin (haldi), azadirachtin (neem), vitamin C and antacid (ranitidine).

 

Essential Readings: 
  1. Biochemistry, Sixth Edition; J.M. Berg, J.L. Tymoczko, & L. Stryer; W.H. Freeman and Co., 2006.
  2. Principles of Biochemistry, Fourth Edition; D.L. Nelson, M.M. Cox, & A.L. Lehninger; W.H. Freeman and Co., 2009.

 

References: 
  1. Harper’s Illustrated Biochemistry; XXVIII edition; R.K. Murray, D.K. Granner, P.A. Mayes, & V.W. Rodwell, Lange Medical Books/ McGraw-Hill, 2009.

e-Resources:

  1. https://www.slideshare.net/mmravagnan/powerpoint-enzymes (Unit II)
  2. https://epgp.inflibnet.ac.in/Home/ViewSubject?catid=MNhNzp1RQlU+6LM40KjY1Q== (Unit I and III)

 

Academic Year: