Biomolecules and Bio-Organic Chemistry

Paper Code: 
24CHY425 B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to

endow with the knowledge of the structure-function relationship of biomolecules, and their importance with regard to maintenance and perpetuation of living systems.

 

Course Outcomes: 

Course

Learning outcome

(at course level)

Learning and Teaching Strategies

Assessment Strategies

Course Code

Course

Title

 

24CHY425 B

 

Biomolecules and Bio-Organic Chemistry (Theory)

CO179:Discuss the properties and functions of enzymes and outline mechanisms of enzyme catalyzed reactions.

CO180:Describe major pathways of carbohydrate metabolism.

CO181:Recognize the structures and functions of amino acids and proteins.

CO182:Analyse the different roles of fat and describe the structure, biosynthesis, oxidation and storage of fatty acids.

CO183:Learn the structure and functions of  RNA and DNA and interpret the structure-function relationships of the proteins, carbohydrates, lipids, and nucleic acids.

CO184:Contribute effectively in course-specific interaction

Approach in teaching:

Interactive lectures, tutorials, group discussions and e-learning.

 

Learning activities for the students:

Peer learning, e- learning, problem solving through tutorials and group discussions.

 

 

Written examinations,

Assignments, Quiz

 

 

14.00
Unit I: 
Enzymes

Remarkable properties of enzymes like catalytic power, specificity and regulation, nomenclature and classification. Fischer's lock and key and Koshland's induced fit hypothesis. Enzyme kinetics: Michaelis-Menten and Lineweaver-Burk plots, Bisubstrate reactions. Enzyme inhibition. Factors affecting enzyme catalysis. Examples of some typical enzyme mechanisms for Chymotrypsin, Ribonuclease, Lysozyme, & Carboxipeptidase A.

Affinity labeling and enzyme modification by site-directed mutagenesis.

 

12.00
Unit II: 
Carbohydrate Metabolism

Structure & classification of carbohydrates. Glycolysis, fate of pyruvate under anaerobic conditions, citric acid cycle, oxidative phosphorylation (electron transport system), gluconeogenesis and glucogenolysis, C4 pathway, pentose phosphate pathway and photosynthesis: C3, C4 & CAM pathway.

 

12.00
Unit III: 
Protein Metabolism and Disorders

Classification of amino acids. Degradation of amino acids (C3, C4, C5 family), urea cycle, uric acid and ammonia formation. Proteins (Structure and Functions): primary, secondary, tertiary and quaternary structure. Extraction and purification techniques.

 

12.00
Unit IV: 
Lipids

Classification and nomenclature of Fatty acids, Classification, Structure and functions of lipids. Biosynthesis of saturated and unsaturated fatty acids, Ketone bodies, membrane lipids-cholesterol, phospholipid and glycolipid, biosynthesis of fat soluble vitamins, biosynthesis of Eicosonoids (prostaglandin, leucotriens and thromboxane). Metabolism of Lipid and fat bodies: Beta-oxidation and channelling of the products to ATP production: minor pathway of fatty acid oxidation: alpha and omega oxidation.

 

10.00
Unit V: 
Nucleic Acids

Chemical and enzymatic hydrolysis, structure and functions of DNA, RNA (m-RNA, t-RNA, r-RNA), an overview of gene expression (replication, transcription and translation), genetic code (origin, Wobble hypothesis and other important features).

 

Essential Readings: 
  1. Biochemistry, Second edition; D.Voet and J.G.Voet; John Wiley & Sons, 1995.
  2. Voet's Principles of Biochemistry, Global Edition; D.Voet, J. G. Voet, C. W. Pratt ;  Wiley, 2018
  3. Lehninger Principles of Biochemistry International Edition, Seventh Edition; D.L. Nelson and Michael Cox; W.H. Freeman & Co.; 2017.

 

References: 
  1. Bioorganic Chemistry: A Chemical Approach to Enzyme Action, Third Edition; H. Dugas; Springer, New York,2012.
  2. Biochemistry, Ninth Edition; L. Stryer; W.H. Freeman & Company, 2019.
  3. Biochemistry and Molecular Biology, third edition; W.H. Elliot and Daphne C. Elliot; Oxford University Press, 2005
  4. Principles and Techniques in Biochemistry and Molecular Biology, sixth edition; K. Wilson and J.Walker; Cambridge University Press, 2007.

 

e-Resources:

  1. Enzymes: principles and biotechnological applications - PMC (nih.gov)
  2. Enzyme databases - PubMed (nih.gov)
  3. Enzymes - an overview | ScienceDirect Topics
  4. Enzyme (genome.gov)

 

Academic Year: