Chemistry Laboratory I

Paper Code: 
24CCHY102
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objective(s):

This course will enable the students to –

get acquainted with various safety measures, detection of extra elements, chromatographic techniques and use of apparatus used in volumetric analysis with correct titrimetric procedure.

Course Outcomes (COs):

Course

Learning Outcome

(at course level)

Learning and Teaching Strategies

Assessment Strategies

Course Code

Course

Title

 

24CCHY 102

 

Chemistry Laboratory I

   (Practical)

CO7: Apply the knowledge of lab safety during the experimental work.

CO8:  Develop the analytical skills to carry out different types of volumetric analysis.

CO9: Determine the presence of extra elements in the given organic compound.

CO10: Apply chromatographic technique to separate and identify different components of mixture.

CO11: Compile and report the results efficiently, and develop communications skills.

CO12: Contribute effectively in course-specific interaction.

Approach in teaching:

Power point presentation, laboratory instructions and

demonstrations.

 

Learning activities for the students:

Peer learning, hands-on-learning,

interpretation of data, record writing and learning through virtual labs.

 

 

Conduction of experiments, lab records, viva voce and quiz.

 

Unit I: 

Laboratory Safety measures                                                                                     (6 Hours)

Handling of hazardous chemicals, incompatible chemicals, flammable solvents, fire hazards in chemical laboratory, control of fire, fire extinguishers, toxicity of chemicals, forms of toxic materials, personal protective equipment, health effects and first aid, MSDS (material safety data sheet), use of compressed gases, waste minimization strategies and chemical waste disposal, procedures for neutralization of strong acids and strong bases.

 

Volumetric Analysis                                                                                                   (30 Hours)

Theoretical aspects-classification and primary requirements of titrations.

Acid-base titrations- basic principle, neutralisation indicators –types, theories of indicators, choice of indicators, titration curves and applications.                                                  

Redox titrations-principle, indicators, and applications of permangnatometry, dichromatometry, iodometry titrations.

Complexometric titration-reactions and properties of EDTA, types of EDTA titrations.

Calibration of fractional weights, pipettes and burettes, preparation of standard solutions, dilution – 0.1 M to 0.001 M solutions, buffer solutions.

  1. Estimation of sodium carbonate and sodium hydrogen carbonate present in a mixture.
  2. Estimation of oxalic acid by titrating it with KMnO4.
  3. Estimation of water of crystallization in Mohr’s salt by titrating with KMnO4.
  4. Estimation of Fe (II) ions by titrating it with K2Cr2O7 using internal indicator.
  5. Estimation of Cu (II) ions iodometrically using Na2S2O3
  6. Estimation of total hardness of water sample

 

Organic Chemistry Experiments                                                                               (24 Hours)

  1. Detection of extra elements (N, S, Cl, Br, I) in organic compounds (containing upto two extra elements)
  1. Separation of mixtures by chromatography: Measure the Rf value in each case  (combination of two compounds to be given)

  1. Identify and separate the components of a given mixture of 2 amino acids (glycine, aspartic acid, glutamic acid, tyrosine or any other amino acid) by paper chromatography

  1. Identify and separate the sugars present in the given mixture by paper chromatography

 

Scheme of Examination

 

The duration of examination will be 4 hrs.

The following exercises will be set in the examination –

Quiz based on lab safety measures                                 10 marks

Volumetric Analysis                                                        30 marks

Organic Chemistry                                                         18 marks

Viva                                                                              12 marks

Total                                                                            70 marks

 

1 Quiz based on Lab Safety Measures

The Quiz will consist of 10 objective type questions (Multiple Choice Questions/Fill in the blanks/One-word answer/True or False/Match the following etc.) carrying 1 mark each. Duration of the quiz will be 10 minutes.

 

2 Volumetric Analysis

The exercise carries 30 marks. The distribution of marks will be as follows:

6 marks will be reserved for the preparation of solutions, neat methodical and systematic working of the candidate.

The remaining 24 marks will be distributed as follows:

  • Theory                                                                          4 marks
  • Correct observation and proper recording                       6 marks
  • Use of correct formula and calculation                            6 marks
  • Result                                                                           8 marks

 

% Error

Marks awarded

≤1.0

8

>1.0 & <1.5

6

>1.5 & <2

4

>2

0
 

 

  1. Organic Chemistry Experiments
  1. Detection of extra elements                                          8 marks
  2. Chromatographic Techniques:

One chromatographic separation of 10 marks will be given. The distribution of marks is as follows:

  • Theory and Procedure                                                  2 marks
  • Preparation of solutions                                               3 marks
  • Observation                                                                 3 marks
  • Result                                                                         2 marks

 

  1. Viva

Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understood the basic principles.

 

Essential Readings: 
  1. Advanced University Practical Chemistry, Part I, First Edition; P.C. Kamboj, Vishal Publishing House, New Delhi; 2016.

 

References: 
  1. Vogel’s Textbook of Quantitative Chemical Analysis; Sixth Edition; M. Thomas, B. Sivasankar, J. Mendham, R.C. Denney, J. D. Barnes; Pearson Education, New Delhi, 2009
  2. Textbook of Practical Organic Chemistry, Fifth edition; Vogel, A.I., Tatchell, A.R., Furnis, B.S., Hannaford, A.J. & Smith, P.W.G., Prentice-Hall, 1996.

e-Resources:

  1. https://vlab.amrita.edu/index.php?sub=2&brch=191&sim=344&cnt=1 (Element Detection)
  2. https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Supplemental_Modules_(Analytical_Chemistry)/Quantifying_Nature/Volumetric_Chemical_Analysis_(Shiundu) (Volumetric Analysis)
  3. https://www.selfstudys.com/books/cbse-lab-manual/english/12th/11-volumetric-analysis/7159 (Volumetric Analysis)
  4. https://egyankosh.ac.in/simple-search?query=titration (Volumetric Analysis)
  5. https://egyankosh.ac.in/bitstream/123456789/43262/1/Unit-5.pdf (Lab Safety)

 

Academic Year: 
2024-2025
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