Chemistry Laboratory III

Paper Code:

25CCHY 213

Credits:

Contact Hours:

60.00

Max. Marks:

100.00

Objective:

This course will enable the students to

synthesize some organic compounds, purify them through crystallization and to inspect the purity through melting point and boiling point along with thin layer & paper chromatography.

Course Outcomes:

Course	Learning Outcome (at course level)	Learning and Teaching Strategies	Assessment Strategies
Course Code	Course title
25CCHY 213	Chemistry Laboratory III (Practical)	CO33: Synthesize organic compounds and, purify them using various solvents, including water, alcohol, and alcohol-water mixtures for crystallization. CO34: Determine melting and boiling points of compounds using distillation and capillary methods and evaluate the effect of impurities. CO35: Separate the components of a given mixture using paper and thin layer chromatography (TLC). CO36: Compile and report the results efficiently, and develop communications skills. CO37: Contribute effectively in course-specific interaction.	Approach in teaching: Power point presentation, laboratory instructions and demonstrations. Learning activities for the students: Peer learning, hands-on-learning, interpretation of data, record writing and learning through virtual labs.	Conduction of experiments, lab records, viva voce.

Course

Learning Outcome

(at course level)

Learning and Teaching Strategies

Assessment Strategies

Course Code

Course

title

25CCHY 213

Chemistry Laboratory III

(Practical)

CO33: Synthesize organic compounds and, purify them using various solvents, including water, alcohol, and alcohol-water mixtures for crystallization.

CO34: Determine melting and boiling points of compounds using distillation and capillary methods and evaluate the effect of impurities.

CO35: Separate the components of a given mixture using paper and thin layer chromatography (TLC).

CO36: Compile and report the results efficiently, and develop communications skills.

CO37: Contribute effectively in course-specific interaction.

Approach in teaching:

Power point presentation, laboratory instructions and demonstrations.

Learning activities for the students:

Peer learning, hands-on-learning, interpretation of data, record

writing and learning through virtual labs.

Conduction of experiments, lab records, viva voce.

Unit I:

Organic Synthesis

· Acetylation of aniline using green approach

· Bromination of acetanilide by conventional method/green approach

· Nitration of acetanilide by conventional method

· S-Benzylisothiouronium salt of of benzoic acid and oxalic acid.

Purification of organic compounds

· Crystallization of synthesized organic compounds using the following solvents: a. Water b. Alcohol c. Alcohol-Water

· Determination of the melting points of above compounds and unknown organic compounds (electrically heated melting point apparatus)

· Effect of impurities on the melting point: mixed melting point of two unknown organic compounds

· Determination of boiling point of liquid compounds. (boiling point lower than and more than 100 °C by distillation and capillary method)

Chromatography

Theory: Classification, principle and working of Thin Layer and Paper chromatography.

Experiments:

· Separation of a mixture of two amino acids by ascending and horizontal paper chromatography.

· Separation of a mixture of two sugars by ascending paper chromatography.

· Separation of a mixture of o-and p-nitrophenol or o-and p-aminophenol by thin layer chromatography (TLC)

Essential Readings:

1. Practical Organic Chemistry, Mann, F.G. & Saunders, B.C., Pearson Education, 2009

References:

1. Advanced Practical Organic Chemistry, Jag Mohan, Himalaya Publishing House ,1992.

2. Vogel’s Textbook of Practical Organic Chemistry, Fifth Edition; B.S. Furniss, A.J. Hannaford, P.W.D. Smith, A.R. Tatchell; Pearson Education, New Delhi, 2003.

e-Resources:

1. https://www.slideshare.net/LavakusaBanavatu/thin-layer-chromatography-43293607 (TLC)

2. https://www.studocu.com/in/u/23692705?sid=01684403081 (all the experiments)

3. https://www.slideshare.net/msaltyy/paper-chromatography-65615443 (Paper Chromatography)

Academic Year:

2025-2026