Chemistry Laboratory XI(B)

Paper Code: 
24DCHY516(B)
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to

  • understand the principles and applications of various separation techniques, including chromatography, solvent extraction, and ion exchange, and apply them to separate and identify compounds in different types of mixtures.
  • perform various analytical techniques, including spectrophotometry, flame photometry, and ion exchange chromatography, to determine the properties of different samples, such as pH and metal ion concentrations.

 

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and Teaching Strategies

Assessment Strategies

Course Code

Course

title

 

24DCHY516(B)

 

 

 

Chemistry Laboratory XI(B)

(Practical)

 

CO128: Perform and interpret the results of various separation techniques, and apply them to separate and identify compounds in mixtures.

CO129: Operate various analytical instruments, to determine the pH, nutrient content, and other parameters of various samples, and interpret the results.

CO130: Identify and apply appropriate separation techniques and analytical methods for different types of samples.

CO131: Compile and report the results efficiently, and develop communications skills.

CO132: Contribute effectively in course-specific interaction.

Approach in teaching:

Power point presentation, laboratory instructions and demonstrations.

 

 

Learning activities for the students:

Peer learning, hands-on-learning, interpretation of data, record

writing and learning through virtual labs.

 

Conduction of experiments, lab records, viva voce.

 

 

 

Unit I: 

Major Experiments

  • To separate a mixture of Ni2+ & Fe2+ by complexation with DMG and extracting the Ni2+- DMG complex in chloroform and determine its concentration by spectrophotometry.
  • Solvent extraction of zirconium with amberliti LA-1, separation from a mixture of iron and gallium.
  • Determination of Na, Ca, Li in cola drinks and fruit juices using flame photometric techniques.
  • Estimation of calcium, magnesium, phosphate, nitrate in soil sample.
  • Determine the composition of the Ferric-salicylate/ ferric-thiocyanate complex by Job’s method.
  • Determination of exchange capacity of cation exchange resins and anion exchange resins.
  • Separation of metal ions from their binary mixture using ion exchange chromatography.
  • Separation of amino acids from organic acids by ion exchange chromatography.

 

Minor Experiments:

  • Determination of pH of soil sample and estimate total soluble salt in this soil sample.
  • To determine the specific conductivity of soil sample.
  • Chromatographic separation (TLC) and identification on the basis of Rf values of -
  • Mixture of Sudan Yellow and Sudan Red (Azo dyes)
  • Active ingredients of plants, flowers, fruit juices and identify them on the basis of their Rf values.
  • Mixture of organic compounds

 

Demonstrations

  •  Determination of dissolved oxygen in water.
  •  Determination of chemical oxygen demand (COD).
  •  Determination of biological oxygen demand (BOD).

 

Scheme of Examination

 

The duration of examination will be 5 hrs.

The following exercises will be set in the examination

Major Experiment                                                                   38 marks

Minor Experiment                                                                   20 marks

Viva                                                                                       12 marks

Total                                                                 70 marks

 

The students will be allotted two experiments to perform in the lab. The exercises can be modified by the examiners and the same will be explained to the candidates. However, such modifications will not involve any departure from the basic principles over which experiments are prescribed in the syllabus.

Candidates shall be given one major and one minor exercise out of the exercises prescribed in the syllabus. The distribution of marks will be as follows:

 

                                                            Major                                      Minor

Theory and procedure                           8 marks                                    4 marks

Correct observation                               8 marks                                    5 marks

Use of correct formula                           8 marks                                    4 marks

Calculation                                            8 marks                                    5 marks

Result                                                   6 marks                                    2 marks

No marks for calculation will be awarded to a candidate for not calculating result.

The permissible error will depend on the nature of the experiment. One mark shall be deducted for each + 2% error over permissible limit.

 

Viva

Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understanding the basic principles.

 

 

Essential Readings: 
  1. Advanced Practical Physical Chemistry, Thirtieth Edition; J. B. Yadav; Krishna Prakashan Media Pvt. Ltd., Meerut, 2015.
  2. Advanced University Practical Chemistry, Part I, First Edition; P.C. Kamboj, Vishal Publishing House, New Delhi; 2013.

 

References: 
  1. Instrumental Methods of Analysis; Seventh Edition; Willard, Hobert H. et. al, CBS, 2004.
  2. Laboratory Hand Book of Chromatographic & Allied Methods; Mikes, O. &Chalmes, R.A; Halsted Press; 1979.
  3. Analytical Chemistry-Methods of Separation; Ditts, R.V.; Van Nostrand, 1974.

 

e-Resources:

 

  1. https://vlab.amrita.edu/index.php?sub=2&brch=193&sim=1315&cnt=1 (soil conductivity)
  2. https://vlab.amrita.edu/index.php?sub=2&brch=193&sim=1549&cnt=1 (soil pH)
  3. https://vlab.amrita.edu/?sub=2&brch=190&sim=338&cnt=1 (spectrophotometry)
  4. https://www.academia.edu/34558723/EXPERIMENT_3_KINETICS_OF_A_SECOND_ORDER_REACTION (kinetics)
  5. https://nptel.ac.in/courses/104106121(chromatography)

 

Academic Year: