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CHEMISTRY LABORATORY XIV
Course Objective(s):
This course will enable the students to –
apply the fundamental concepts/theory of different instrumental methods and analytical techniques used in chemistry.
Course Outcomes (COs):
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Course Outcomes |
Teaching Learning Strategies |
Assessment Strategies |
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On completion of this course, the students will be able to- CO126: determine the amount of cations, vitamin C using UV spectrophotometer. CO127: interpret IR, NMR spectra of various organic compounds. CO128: apply chromatographic technique for separation of organic compounds. CO129: analyse of data using a spreadsheet program such as excel, origin. |
instructions and demonstrations
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A list of experiments are given below. Students are required to perform 8-10 experiments.
- To verify the Beer-Lambert law for a compound (Potassium permanganate, copper sulphate, methylene blue etc.) and determine the concentration of the substance using calibration curve.
- Detection of adulteration of food stuffs - To detect the total dye content in sunset yellow colour.
- Determination of a Mixture of Cobalt and Nickel (UV/Vis spec.)
- Study of Electronic Transitions in Organic Molecules (i.e., acetone in water and related system)
- Determination of vitamin C in fruit.( (UV/Vis spec.)
- Determine the composition of the Fe3+-salicylic acid/ iron-phenanthroline complex solution by Job’s method/mole ratio method.
- To titrate Fe(II) with KMnO4 spectrophotometrically.
- To determine the concentration of Fe (II) by spectrophotometric titration with EDTA.
- To Titrate copper solution with EDTA spectrophotometrically.
- Determination of the isoelectric pH of a protein.
- Titration curve of an amino acid.
- Interpretation of IR and NMR spectra of simple organic compounds (ethanol, 1-propanol, 2-propanol, methylethanoate, ethyl acetate,stilbene, ethyl acetate, propanone etc).
- IR Absorption Spectra (Study of Aldehydes and Ketones in different solvents)
- Determination of Calcium, Iron, and Copper in Food by Atomic Absorption
- Separation of Carbohydrates by HPLC/Paper chromatography
- Determination of Caffeine in Beverages by HPLC
- Potentiometric Titration of a Chloride-Iodide Mixture
- Cyclic Voltammetry of the Ferrocyanide/Ferricyanide Couple
- Determination of the void volume of a gel filtration column.
- Use of fluorescence to do “presumptive tests” to identify blood or other body fluids.
- Use of “presumptive tests” for anthrax or cocaine
- Use of capillary electrophoresis with laser fluorescence detection for nuclear DNA (Y chromosome only or multiple chromosome)
- Quantitative Analysis of Mixtures by Gas Chromatography (i.e., chloroform and carbon tetrachloride)
- Laboratory analysis to confirm anthrax or cocaine
- Detection in the field and confirmation in the laboratory of flammable accelerants or explosives
- Detection of illegal drugs or steroids in athletes
- Detection of pollutants or illegal dumping
- Fibre analysis
Any other experiment related to topic carried out in the class.
- Advanced Practical Physical Chemistry, Eighteenth Edition; J.B. Yadav; Goel Publishing House, Meerut, 2015.
- Principles of Instrumental Analysis, Seventh Edition; Skoog, D.A., Holler F.J. and S.R. Crouch; Cengage Learning, USA, 2016.
SUGGESTED READINGS:
- Experimental Physical Chemistry, First Edition; V. D. Athawale, P. Mathur; New Age International (P) Ltd. Publishers, New Delhi, 2011.
- Advanced University Practical Chemistry, Part I, First Edition; P.C. Kamboj, Vishal Publishing House, New Delhi; 2016.
e-Resources:
- https://egyankosh.ac.in/bitstream/123456789/79539/1/Unit-5.pdf (Job’s method)
- https://vlab.amrita.edu/?sub=3&brch=63&sim=1336&cnt=1#:~:text=Titration%20curves%20are%20obtained%20when,per%20mole%20of%20the%20sample. (Titration curve of amino acid)
- https://vlab.amrita.edu/?sub=2&brch=190&sim=338&cnt=1 (UV)
Scheme of Examination
The duration of examination will be 5 hrs.
The following exercises will be set in the examination:
Physical experiment 58 marks
Viva 12 marks
Total 70 marks
The exercise carries 58 marks. The candidates shall be given either one exercise of 58 marks or two exercise of 29 marks each out of exercises prescribed in the syllabus. The exercises in physical chemistry can be modified by the examiner and the same will be explained to the candidates. However, such modifications will not involve any departure from the basic principles over which experiments are prescribed in the syllabus.
The distribution of marks will be as follows
Theory and procedure 8 marks
Correct observation 18 marks
Use of correct formula 8 marks
Calculation 16 marks
Result 8 marks
Viva Oral questions shall generally be asked over the work assigned to the candidate. The purpose is tofind out as to what extent the candidate has understanding the basic principles.
