INSTRUMENTAL METHODS OF CHEMICAL ANALYSIS

Paper Code: 
D-CHY 613 B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objective(s):

This course will enable the students to –

understand the fundamental concepts/theory and application of different instrumental methods and analytical techniques used in chemistry.

 

Course Outcomes (COs):

Course Outcomes

Teaching Learning

Strategies

Assessment

Strategies

On completion of this course, the students will be able to-

CO112:  explain instrumentation part of IR, UV-Vis spectrometer.
CO113: describe the basic concept of mass spectrometry including different ionization processes of samples.

CO114: apply different chromatographic techniques in the separation & identification of components of a system.

CO115: acquaint with the instrumental technique of elemental analyses.

CO116: describe basic principles involved in NMR, electro analytical, surface imaging technique.
  • Interactive lectures
  • Tutorials
  • Group discussions
  • Digital learning

 
  • Oral and written examinations
  • Assignments
  • Quiz

 

14.00
Unit I: 
Molecular Spectroscopy

Infrared spectroscopy: Interactions with molecules, absorption and scattering, means of excitation (light sources), separation of spectrum (wavelength dispersion, time resolution), detection of the signal (heat, differential detection), interpretation of spectrum (qualitative, mixtures, resolution), advantages of Fourier Transform (FT IR), applications- issues of quality assurance and quality control, special problems for portable instrumentation and rapid detection.

UV-Visible/ Near IR: Introduction to emission, absorption, fluorescence and photoaccoustic. Excitation sources (lasers, time resolution), wavelength dispersion (gratings, prisms, interference filters, laser, placement of sample relative to dispersion, resolution), detection of signal (photocells, photomultipliers, diode arrays, sensitivity and S/N), single and double beam instruments, interpretation (quantification, mixtures, absorption vs. fluorescence and the use of time, photoaccoustic, fluorescent).

10.00
Unit II: 
Mass Spectroscopy

Methods of making the gaseous molecule into an ion (electron impact, chemical ionization method), methods of making liquids and solids into ions (electrospray, electrical discharge, laser desorption, fast atom bombardment), mass analyzers (magnetic-deflection, time of flight, electric quadrupole), resolution, ion- collection systems, detection and interpretation.

11.00
Unit III: 
Separation Techniques

Chromatography: Introduction of gas chromatography, liquid chromatography, supercritical fluids chromatography.

Importance of column technology (packing, capillaries), factors affecting separation (volatility, solubility, interactions with stationary phase, size, electrical field), detection: simple vs. specific (gas and liquid), detection as a means of further analysis (use of tags and coupling to IR and MS), Electrophoresis (plates and capillary) and use with DNA analysis.

11.00
Unit IV: 
Elemental Analysis Techniques

Atomic spectroscopy: Atomic absorption, Atomic emission, and Atomic fluorescence.

Excitation and methods of getting sample into gas phase (flames, electrical discharges, plasmas), wavelength separation and resolution (dependence on technique), detection of radiation (simultaneous/scanning, signal noise), interpretation (errors due to molecular and ionic species, matrix effects, other interferences).

14.00
Unit V: 
Miscellaneous Techniques

Instrumentation and applications - NMR spectroscopy, electroanalytical methods-potentiometry & voltammetry, radiochemical Methods, X-ray analysis and electron spectroscopy (surface analysis) .

Essential Readings: 
  • Principles of Instrumental Analysis, Seventh Edition; Skoog, D.A., Holler F.J. and S.R. Crouch; Cengage Learning, USA, 2016.
  • Instrumental Methods of Analysis, Seventh Edition; H.H.  Willard, L.L. Merritt, J.A. Dean, F.A. Settle CBS Publishers, New Delhi, 2004

 

References: 

SUGGESTED READINGS:

  • Fundamentals of Molecular Spectroscopy, Fourth Edition; C. N. Banwell and E. M. Mc Cash; Tata McGraw Hill, New Delhi, 2017.
  • Basic Concepts of Analytical Chemistry, Fourth Edition; Khopkar, S.M., New Age International Pvt Ltd, New Delhi, 2020.

e-RESOURCES:

 

Academic Year: 
2023-2024
  • Printer-friendly version
  • PDF version