Analytical Biochemistry

Paper Code: 
BCH 122
Contact Hours: 
90.00
18.00
Unit I: 
Spectroscopic Techniques

Basic principles, instrumentation and applications of UV-visible, IR, fluorimetry, atomic absorption and emission spectrophotometry. Basic principles, instrumentation and applications of ESR, NMR and mass spectroscopy (EI,CI, MALDI-TOF). Biochemical applications of fluorescence, emission, NMR and mass spectroscopy.

18.00
Unit II: 
Radioactivity and Radioisotopic Techniques

Radioactivity, Stable and radioactive isotopes, concepts of half life and decay, principles of scintillation counting, GM counters, applications of isotopes, Isotope dilution technique, autoradiography, turnover studies, precursor-product relationship, production of radio-labelled biomolecules, calculations involving isotopes, radiation hazards and methods for contaminant prevention. Nature of radioactivity, properties of α, β and γ-rays, measurement of radioactivity, use of radioisotopes in research, In vivo and in vitro labelling techniques, double labelling, quenching, internal standard, channel ratio, external standard ratio, emulsion counting, radioactive decay.

18.00
Unit III: 
Sedimentation based techniques for isolation and purification of biological samples

Principles of centrifugation, concepts of RCF, different types of instruments and rotors, preparative, differential and density gradient centrifugation, analytical ultracentrifugation, determination of molecular weights and other applications, the oxygen electrode, Organ perfusion, tissues homogenates in the study of intermediary metabolism. Use of various isotopes in metabolic studies.

18.00
Unit IV: 
Electrophoresis

Principles of electrophoretic separation, zonal and continuous electrophoresis, paper, cellulose acetate/nitrate, gel and capillary electrophoresis, use of native and denaturating gels, Isoelectric focussing and two dimensional gel electrophoresis, electroporation, pulse field gel electrophoresis, Gradient gels.

18.00
Unit V: 
Chromatography and X-ray Crystallography

Pre requisites- Paper and thin layer chromatography. Chromatography, principles of adsorption, partition and ion-exchange chromatography, gel permeation chromatography, GC , GC-MS and HPLC. X-ray Crystallography - protein crystals, Bragg's law, unit cell, isomorphous replacement, fiber pattern of DNA.

Essential Readings: 
  1. Lehninger's Principles of Biochemistry, Second edition, D L Nelson and M M Cox, Macmillan Worth Pub. Inc. New York, 2009.
  2. Principles and Techniques of Practical Biochemistry, Fourth edition, K Wilson and J Walker (eds.) Cambridge Univ.Press, 1999.
  3. Physical Biochemistry, Second edition, David Friefelder, W H Freeman and Co., New York, 2008.
  4. Outlines of Biochemistry, Eric E Conn, P K Stumpf, G Bruening and Roy H Doi , John Wiley & Sons,New York, 2007.
  5.  Vogel’s Textbook of Quantitative Chemical Analysis; Fifth Edition; G.H. Jeffery, J. Bassett. J. Mendham, R.C. Denney; Longman Scientific and Technical Publication, England, 1991.
  6.  Fundamentals of Analytical Chemistry, Seventh Edition; D.A. Skoog, D.M. West and F.J. Holler; Saunders College Publishing Philadelphia, 1991.
  7. Quantitative Analysis, Sixth Edition; R.A. Day, A.L. Underwood; Prentice-Hall of India Pvt. Ltd., New Delhi, 1999.
  8. Textbook of Biochemistry, Fourth edition, West and Todd, Oxford and IBH Publishing Co., 1974.
  9. Enzymes, Biochemistry, Biotechnology and Clinical Chemistry, First edition, Trevor Palmer, Harwood Publishing Ltd., 2001.

 
 

Academic Year: 
2018-2019
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