Course Objectives:
This course will enable the students to -
understand the concept of basic analytical techniques.
discuss optical methods of analysis and chromatographic separation techniques.
Course Outcomes (COs):
Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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CHY-414 |
Analytical Chemistry
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The students will be able to –
CO114: understand basic concepts and applications of Potentiometry, Electrogravimetry, Coulometry and solvent extraction. CO115: explain the applications of Polarography and Polarimetry methods in quantitative and qualitative estimation. CO116: illustrate various optical methods of analysis such as Spectrophotocolorimetry, Atomic absorption spectroscopy and Flame Photometry CO117: explain the significance of Rf value. CO118: understand the principle and applications of various Chromatographic separation techniques like gas, liquid and ion exchange chromatography. |
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Potentiometry– Basic concepts and Applications in Acid-base titrations, oxidation-reduction titrations, precipitation titrations and complexometric titrations.
Electrogravimetry-Basic concept: current density, polarization, decomposition voltage, electrolysis at constant current and constant voltage and its basic applications.
Coulometry-Basic concepts: Constant current coulometry, constant potential coulometry, applications in neutralization titration, precipitation titration, oxidation- reduction titration.
Polarography – Introduction, polarographic measurement, half wave potential, residual current, migration current, diffusion current, Ilkovic equation, polarographic cell and dropping mercury electrode. Precautions and advantages, applications in quantitative and qualitative analysis.
Polarimetry- polarization of light, optical activity, angle of rotation, specific rotation, measurement of rotatory power (instrumentation), application of polarimetry in quantitative and qualitative analysis.
Solvent extraction- distribution law, process of extraction (liquid- liquid, solid-liquid), techniques for solvent extraction, classification and applications.
Pre requisite: Lambert and Beer law, verification, derivation, signification of λmax and molar absorptivity, theory of fluorescence and phosphorescence.
Spectrophotocolorimetry: Single beam and double beam spectrophotometers, functions of the components, applications.
Atomic absorption spectroscopy: Principle, instrumentation, spectral interferences and chemical interferences in atomic absorption spectroscopy, applications in quantitative analysis (analysis of Zn2+, Cu2+ and Pb2+).
Flame photometry: Principle, instrumentation, interferences in flame photometry, applications in quantitative analysis. Comparison of atomic absorption and flame emission spectroscopy.
General description and classification of chromatographic methods, thin layer, paper and column chromatographic techniques and their simple applications, Rf-values and their significance, elution in column chromatography, migration rates of solutes, band broadening and column efficiency, column resolution.
Principle, instrumentation, and applications of gas chromatography, high performance liquid chromatography and ion exchange chromatography.