Analytical Methods in Chemistry

Paper Code: 
24DCHY501(B)
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to

understand the concept of basic analytical techniques and interpret the data applying statistical methods.

 

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and Teaching Strategies

Assessment Strategies

Course Code

Course

Title

 

24DCHY 501(B)

 

 

Analytical Methods in Chemistry

(Theory)

 

CO60: Discuss the principles and applications involved in pH metric, potentiometric and conductometric titrations.

CO61: Describe the concepts and applications of thermogravimetry and solvent extraction.

CO62: Illustrate fundamental laws of spectroscopy and explain the principles, instrumentation and applications of UV-visible spectrometry.

CO63: Explain the principle, instrumentation and applications of infrared spectrometry, atomic absorption and emission spectroscopy.

CO64: Describe the principle and applications of various chromatographic separation techniques and apply it for stereoisomeric separation and analysis.

CO65: Contribute effectively in course-specific interaction.

Approach in teaching:

Interactive lectures, tutorials, group discussions and e-learning.

 

Learning activities for the students:

Peer learning, e- learning, problem solving through tutorials and group discussions.

 

 

Written examinations,

assignments and quiz.

 

 

 

 

12.00
Unit I: 
Basic Analytical Techniques I

Qualitative and quantitative aspects of analysis: Sampling, evaluation of analytical data, errors, accuracy and precision, methods of their expression, normal law of distribution if indeterminate errors, statistical test of data; F, Q and t-test, rejection of data, and confidence intervals.

Electroanalytical methods: Classification of electroanalytical methods, basic principle and applications of pH metric, potentiometric and conductometric titrations. Techniques used for the determination of equivalence points and pKa values.

 

12.00
Unit II: 
Basic Analytical Techniques II

Thermal methods of analysis: Theory of thermogravimetry (TG), basic principle of instrumentation. Techniques for quantitative estimation of Ca and Mg from their mixture.

Solvent extraction: Classification, principle and efficiency of the technique. Mechanism of extraction: extraction by solvation and chelation.

Techniques of extraction-batch, continuous and counter-current extractions.

Qualitative and quantitative aspects of solvent extraction- extraction of metal ions from aqueous solution, extraction of organic species from the aqueous and nonaqueous media.

 

11.00
Unit III: 
Optical Methods of Analysis I

Origin of spectra, interaction of radiation with matter, fundamental laws of spectroscopy and selection rules, validity of Beer-Lambert’s law.

UV-Visible Spectrometry: Basic principles of instrumentation (choice of source, monochromator and detector) for single and double beam instruments.Basic principles of quantitative analysis, estimation of metal ions from aqueous solution, geometrical isomers, keto-enol tautomers. Determination of composition of metal complexes using Job’s method of continuous variation and mole ratio method.

 

12.00
Unit IV: 
Optical Methods of Analysis II

Infrared Spectrometry: Basic principles of instrumentation (choice of source, monochromator & detector) for single and double beam instrument; sampling techniques.

Structural illustration through interpretation of data, effect and importance of isotope substitution.

Atomic Absorption and Emission Spectrometry: Basic principles of instrumentation (choice of source, monochromator, detector, choice of flame and burner designs). Techniques of atomization and sample introduction, method of background correction, interferences and their method of removal. Techniques for the quantitative estimation of trace level of metal ions from water samples.

 

13.00
Unit V: 
Separation Techniques

Chromatography: Classification, principle and efficiency of the technique.

Mechanism of separation- adsorption, partition & ion exchange. Development of chromatograms: frontal, elution and displacement methods.

Qualitative and quantitative aspects of chromatographic methods of analysis- IC, GLC, GPC, TLC and HPLC.

Stereoisomeric separation and analysis: Measurement of optical rotation, calculation of enantiomeric excess (ee)/ diastereomeric excess (de) ratios and determination of enantiomeric composition using NMR, chiral solvents and chiral shift reagents. Chiral chromatographic techniques using chiral columns (GC and HPLC).

 

Essential Readings: 
  1. Principles of Instrumental Analysis; Seventh Edition; Skoog, D.A., Holler F.J. and S.R. Crouch; Cengage Learning, USA, 2016.

 

References: 
  1. Instrumental Methods of Analysis, Seventh Edition; Willard, Hobert H. et. al, CBS, 2004.
  2. Analytical Chemistry, Seventh Edition; Christian, Gary D; John Willy New York, 2013.
  3. Basic Concepts of Analytical Chemistry, Fourth Edition; Khopkar, S.M., New Age International Pvt Ltd, 2020.
  4. Analytical Chemistry-Methods of Separation, Ditts R.V.; Van Nostrand, 1974.

 

e-Resources:

  1. https://nptel.ac.in/courses/104106121 (Unit I & V)
  2. https://nptel.ac.in/courses/103108124 (Unit III & IV)
  3. https://nptel.ac.in/courses/104105084 (Unit II)

 

Academic Year: 
2024-2025
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