ANALYTICAL METHODS IN CHEMISTRY

Paper Code: 
D-CHY 501 B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objective(s):
This course will enable the students to -
understand the concept of basic analytical techniques and interpret the data applying statistical methods

Course Outcomes (COs):

Course Outcomes

Teaching Learning Strategies

Assessment

Strategies

  

On completion of this course, the students will be able to-

 

CO45: differentiate between different types of errors and distinguish between accuracy and precision and validate the results.

CO46: discuss principles involved in pH metric, potentiometric and conductometric titrations.

CO47: understand basic concepts and applications of thermogravimetry and solvent extraction.

CO48: illustrate various optical methods of analysis such as UV-Visible Spectrometry, Infrared Spectrometry and Flame Atomic absorption and emission spectroscopy.

CO49: understand the principle and applications of various Chromatographic separation techniques like IC, GLC, GPC, TLC and HPLC

 

·       Interactive lectures

·       Tutorials

·       Group discussions

·       Digital learning

·       Problem solving sessions

·       Assertion and Reasoning

 

·       Oral and written examinations

·       Assignments

·       Quiz

 

 

  

 

12.00
Unit I: 
Basic Analytical Techniques I

Qualitative and quantitative aspects of analysis: Sampling, evaluation of analytical data, errors, accuracy and precision, methods of their expression, normal law of distribution if indeterminate errors, statistical test of data; F, Q and t-test, rejection of data, and confidence intervals.

Electroanalytical methods: Classification of electroanalytical methods, basic principle and applications of pH metric, potentiometric and conductometric titrations. Techniques used for the determination of equivalence points and pKa values.

12.00
Unit II: 
Basic Analytical Techniques II

Thermal methods of analysis: Theory of thermogravimetry (TG), basic principle of instrumentation. Techniques for quantitative estimation of Ca and Mg from their mixture.

Solvent extraction: Classification, principle and efficiency of the technique. Mechanism of extraction: extraction by solvation and chelation.

Techniques of extraction-batch, continuous and counter-current extractions.

Qualitative and quantitative aspects of solvent extraction- extraction of metal ions from aqueous solution, extraction of organic species from the aqueous and nonaqueous media.

11.00
Unit III: 
Optical Methods of Analysis I

Origin of spectra, interaction of radiation with matter, fundamental laws of spectroscopy and selection rules, validity of Beer-Lambert’s law.

UV-Visible Spectrometry: Basic principles of instrumentation (choice of source, monochromator and detector) for single and double beam instruments.Basic principles of quantitative analysis, estimation of metal ions from aqueous solution, geometrical isomers, keto-enol tautomers. Determination of composition of metal complexes using Job’s method of continuous variation and mole ratio method.

12.00
Unit IV: 
Optical Methods of Analysis II

Infrared Spectrometry: Basic principles of instrumentation (choice of source, monochromator & detector) for single and double beam instrument; sampling techniques.

Structural illustration through interpretation of data, effect and importance of isotope substitution.

Atomic Absorption and Emission Spectrometry: Basic principles of instrumentation (choice of source, monochromator, detector, choice of flame and burner designs). Techniques of atomization and sample introduction, method of background correction, interferences and their method of removal. Techniques for the quantitative estimation of trace level of metal ions from water samples.

13.00
Unit V: 
Separation Techniques

Chromatography: Classification, principle and efficiency of the technique.

Mechanism of separation- adsorption, partition & ion exchange. Development of chromatograms: frontal, elution and displacement methods.

Qualitative and quantitative aspects of chromatographic methods of analysis- IC, GLC, GPC, TLC and HPLC.

Stereoisomeric separation and analysis: Measurement of optical rotation, calculation of enantiomeric excess (ee)/ diastereomeric excess (de) ratios and determination of enantiomeric composition using NMR, chiral solvents and chiral shift reagents. Chiral chromatographic techniques using chiral columns (GC and HPLC).

Essential Readings: 
  • Principles of Instrumental Analysis; Seventh Edition; Skoog, D.A., Holler F.J. and S.R. Crouch; Cengage Learning, USA, 2016.

References: 

SUGGESTED READINGS:

  • Instrumental Methods of Analysis, Seventh Edition; Willard, Hobert H. et. al, CBS, 2004.
  • Analytical Chemistry, Seventh Edition; Christian, Gary D; John Willy New York, 2013.
  • Basic Concepts of Analytical Chemistry, Fourth Edition; Khopkar, S.M., New Age International Pvt Ltd, 2020.
  • Analytical Chemistry-Methods of Separation, Ditts R.V.; Van Nostrand, 1974.

e-RESOURCES:

Academic Year: 
2023-2024
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