Biochemical Genetics And Dna Replaction

Human pedigree analysis, Linkage- mapping by recombinant frequency, mapping with molecular markers; Gene interaction- Interaction between the alleles of a single gene- variation or dominance. Basic concepts of Mendelian and non-Mendelian inheritance, importance of meiosis in heredity, sex linked inheritance, polygenic and maternal inheritance,

Genome organization in prokaryotes and eukaryotes, plasmids, transposons, insertion sequences and retroposons, mitochondrial and chloroplast DNA, Benzer's fine structures of rll loci, organization of eukaryotic chromosomes. histones and non-histone type DNA binding proteins, nucleosomes and higher order structures, C-value pradox and the significance of introns, single copy genes,repeating sequencs, and tandem gene clusters, r-RNA genes, histone genes and immunoglobulin genes, selfish DNA, structure, occurrence and biological

Mutants, different kinds of mutations, Isolation of mutants, phage mutants, host range rapidlysis and temperature sensitive mutants, mechanism of mutants, gene transfer mechanisms, transformation, trasduction, (generalized, abortive and specialized), conjugation F+ x F-Hfr strains, mechanism of recombinant and cross over, elements of gene mapping, mapping by recombination analysis, multiple cross over and interference, circular chromosome and mapping by conjugation, tetrad and complement analysis, mapping by transformation and transduction, map units and cytological maps of eukaryotic chromosomes, somatic cell genetics.
DNA repair : Direct reversal of damage, excision repair, recombinant repair,the SOS response, identification of carcinogens, inhibitors of DNA replication.
 

Semiconservative replication, replication forks, role of DNA gyrase, continuous and discontinuous synthesis, evidence for Okazakai model, RNA primers, enzymes in replication, single strand DNA binding proteins, helicases, topoisomerases, DNA primases, DNA ligases, DNA polymerases, E coli DNA polymerases I and II, eukaryotic DNA polymerases, prokaryotic replication mechanisms, rolling cycle replication, replication of E coli DNA. Eukaryotic DNA replication.

Autonomous replicating sequences, yeast plasmid replication (double rolling circle), mitochondrial DNA replication, reverse transcriptase, termination and fidelity of replication, fusion of replicons and termination signals, telomers, inhibition of DNA replication