Coming soon
Biomolecules and Bio-Organic Chemistry
This course will enable the students to
endow with the knowledge of the structure-function relationship of biomolecules, and their importance with regard to maintenance and perpetuation of living systems.
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Course Code |
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25CHY425 B |
Biomolecules and Bio-Organic Chemistry (Theory) |
CO176 Discuss the properties and functions of enzymes and outline mechanisms of enzyme catalyzed reactions. CO177 Describe major pathways of carbohydrate metabolism. CO178 Recognize the structures and functions of amino acids and proteins. CO179 Analyse the different roles of fat and describe the structure, biosynthesis, oxidation and storage of fatty acids. CO180 Learn the structure and functions of RNA and DNA and interpret the structure-function relationships of the proteins, carbohydrates, lipids, and nucleic acids. CO181 Contribute effectively in course-specific interaction |
Approach in teaching: Interactive lectures, tutorials, group discussions and e-learning.
Learning activities for the students: Peer learning, e- learning, problem solving through tutorials and group discussions.
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Written examinations, Assignments, Quiz
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Remarkable properties of enzymes like catalytic power, specificity and regulation, nomenclature and classification. Fischer's lock and key and Koshland's induced fit hypothesis. Enzyme kinetics: Michaelis-Menten and Lineweaver-Burk plots, Bisubstrate reactions. Enzyme inhibition. Factors affecting enzyme catalysis. Examples of some typical enzyme mechanisms for Chymotrypsin, Ribonuclease, Lysozyme, & Carboxipeptidase A.
Affinity labeling and enzyme modification by site-directed mutagenesis.
Structure & classification of carbohydrates. Glycolysis, fate of pyruvate under anaerobic conditions, citric acid cycle, oxidative phosphorylation (electron transport system), gluconeogenesis and glucogenolysis, C4 pathway, pentose phosphate pathway and photosynthesis: C3, C4 & CAM pathway.
Classification of amino acids. Degradation of amino acids (C3, C4, C5 family), urea cycle, uric acid and ammonia formation. Proteins (Structure and Functions): primary, secondary, tertiary and quaternary structure. Extraction and purification techniques.
Classification and nomenclature of Fatty acids, Classification, Structure and functions of lipids. Biosynthesis of saturated and unsaturated fatty acids, Ketone bodies, membrane lipids-cholesterol, phospholipid and glycolipid, biosynthesis of fat soluble vitamins, biosynthesis of Eicosonoids (prostaglandin, leucotriens and thromboxane). Metabolism of Lipid and fat bodies: Beta-oxidation and channelling of the products to ATP production: minor pathway of fatty acid oxidation: alpha and omega oxidation.
Chemical and enzymatic hydrolysis, structure and functions of DNA, RNA (m-RNA, t-RNA, r-RNA), an overview of gene expression (replication, transcription and translation), genetic code (origin, Wobble hypothesis and other important features).
- Biochemistry, Second edition; D.Voet and J.G.Voet; John Wiley & Sons, 1995.
- Voet's Principles of Biochemistry, Global Edition; D.Voet, J. G. Voet, C. W. Pratt ; Wiley, 2018
- Lehninger Principles of Biochemistry International Edition, Seventh Edition; D.L. Nelson and Michael Cox; W.H. Freeman & Co.; 2017.
- Bioorganic Chemistry: A Chemical Approach to Enzyme Action, Third Edition; H. Dugas; Springer, New York,2012.
- Biochemistry, Ninth Edition; L. Stryer; W.H. Freeman & Company, 2019.
- Biochemistry and Molecular Biology, third edition; W.H. Elliot and Daphne C. Elliot; Oxford University Press, 2005
- Principles and Techniques in Biochemistry and Molecular Biology, sixth edition; K. Wilson and J.Walker; Cambridge University Press, 2007.
e-Resources:
- Enzymes: principles and biotechnological applications - PMC (nih.gov)
- Enzyme databases - PubMed (nih.gov)
- Enzymes - an overview | ScienceDirect Topics
- Enzyme (genome.gov)
