Coming soon
CHEMISTRY LAB VII
Course Objective(s):
This course will enable the students to -
- understand the concept of quantitative gravimetric analysis method.
- provide an in-depth knowledge of various chromatographic techniques.
Course Outcomes (COs):
|
Course Outcomes |
Teaching Learning Strategies |
Assessment Strategies |
|
On completion of this course, the students will be able to- CO125: apply quantitative gravimetric analysis method. CO126: estimate percentage and number of functional groups by quantitative analysis method. CO127: separate green leaf pigments by TLC and ascending paper chromatography. CO128: apply circular paper chromatography and column chromatography. |
|
|
Quantitative Analysis: Gravimetric Analysis (Any Three)
a) Estimation of Cu as CuSCN
b) Estimation of Zn as Zinc ammonium phosphate
c) Estimation of Pb as PbCrO4
d) Estimation of Ni as Ni(DMG)2
Quantitative Analysis: Estimation of functional groups
a) Determination of the percentage and number of hydroxyl groups in an organic compounds by acetylation method.
b) Estimation of amines/phenols using bromated/bromide solution.
Chromatography
a) Separation of 2,4 –dinitrophenylhydrazones of acetone, 2-butanone, hexan-2- one and hexane-3-one using toluene and light petroleum (40:60) by TLC
b) Separation of green leaf pigments (spinach leaves may be used) by TLC
c) Separation of green leaf pigments (spinach leaves may be used) by ascending paper chromatography
d) Separation of amino acids or carbohydrates by circular paper chromatography.
e) Separation of mixture of organic compounds by column chromatography
- Vogel’s Textbook of Quantitative Chemical Analysis, Sixth Edition; M. Thomas; B. Sivasankar, J. Mendham; R.C. Denney; J. D. Barnes; Pearson Education, New Delhi, 2009.
SUGGESTED READINGS:
- Vogel’s Textbook of Practical Organic Chemistry, Fifth Edition; B.S. Furniss; A.J. Hannaford; P.W.D. Smith; A.R. Tatchell; Pearson Education, New Delhi, 2003.
e-RESOURCES:
Marking Scheme
The duration of examination will be 4 hrs.
The following exercises will be set in the examinations
- Inorganic Chemistry : Gravimetric analysis 25 marks
- Organic estimation 20 marks
- Organic Chemistry: Chromatographic separation 10 marks
- Viva 15marks
Total 70 marks
Gravimetric analysis
The exercise carries 25 marks. Five marks shall be as awarded to a candidate for a neat, methodical and systematic work.
The remaining 20 marks will be distributed as follows:
- Theory 3 marks
- Correct observation and proper recording 3 marks
- Use of correct formula and calculation 2 marks
- Result 12 marks
|
% Error |
Marks |
% Error |
Marks |
|
1.0 |
12 |
1.7 |
5 |
|
1.1 |
11 |
1.8 |
4 |
|
1.2 |
10 |
1.9 |
3 |
|
1.3 |
9 |
2.0 |
2 |
|
1.4 |
8 |
2.1 |
1 |
|
1.5 |
7 |
2.2 |
0 |
|
1.6 |
6 |
|
|
Chromatographic Techniques:
One chromatographic separation of 10 marks will be given. The distribution of marks is as follows:
- Theory and procedure 2 marks
- Preparation of solutions 3 marks
- Observation 3 marks
- Result 2 marks
Organic chemistry: Quantitative analysis-
The exercise carries: 20 marks.
One quantitative experiment on organic analysis given.
- Theory and procedure 4 marks
- Preparation of solutions 3 marks
- Observation 3 marks
- Calculation 5 marks
- Result 5 marks
Viva:
Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understood the basic principles of chemistry.
