CHEMISTRY LAB X

Paper Code: 
CHY 616
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objective(s):

This course will enable the students to –

  • get an insight on the use of apparatus used in colorimetry like spectrophotometer and to enable them to record absorption spectrum of substances.
  • acquire knowledge of spectrophotometric estimations of proteins, carbohydrates and cholesterols

Course Outcomes (COs):

Course Outcomes

Teaching Learning Strategies

Assessment

Strategies

On completion of this course, the students will be able to-

CO181: apply Beer’s Law, relating solution concentration to absorbance and use the measurement of light absorption (colorimetry) to detect the colorimetric reaction of thiocyanate with iron

CO182: acquire knowledge to determine the spectrophotometric estimations of protein, carbohydrate and cholesterol along with their calculations

CO183: interpret the method of continuous variation to determine the reaction stoichiometry for the formation of a metal ion complex

CO184: learn the technique of absorption spectroscopy in order to monitor the relative concentration of a colored metal ion complex in solution
  • Demonstrations
  • Peer learning
  • Interactive lectures
  • Problem solving exercise,
  • Handouts and manuals

 
  • Viva voce
  • Quiz

 

Unit I: 

Physical Chemistry Experiments

Colorimetry

a) Job’s method of continuous variation by iron-phenanthroline complex.
b) Mole Ratio method by iron-phenanthroline complex.
c) Record an absorption spectrum of a substance (KMnO4/K2Cr2O7) using a spectrophotometer and determine absorption maxima (λmax).
d) To verify the Beer-Lambert law for a compound(Potassium permanganate, copper sulphate, methylene blue etc.) and determine the concentration of the substance using calibration curve.

Polarimeter
e) Determine the rate constant of the inversion of cane sugar in presence of hydrochloric acid and sulphuric acid by using polarimeter and evaluate the relative strength of the two acids.

Spectrophotometric Estimations

  1. Protein
  2. Carbohydrate
  3. Cholesterol

 

Essential Readings: 
  • Vogel’s Textbook of Practical Organic Chemistry, Fifth Edition; B.S. Furniss, A.J. Hannaford, P.W.D. Smith, A.R. Tatchell; Pearson Education, New Delhi, 2003.

 

References: 

SUGGESTED READINGS:

  • Advanced Practical Physical Chemistry, Eighteenth Edition; J.B. Yadav; Goel Publishing House, Meerut, 2015.

e-RESOURCES:

 

Marking Scheme

The duration of examination will be 4 hrs.

The following exercises will be set in the examinations:

  • Physical Experiment (two major experiment)                                27+28 marks
  • Viva                                                                                                15 marks

                                                                        Total                     70 marks

Physical Chemistry Experiment:

The exercise carries 55 marks. Two major experiments of 27 and 28 marks respectively will be given to candidate by lot. Fifteen marks (7 and 8) shall be awarded for preparatory work, setting up of an apparatus, neat and methodical work.

40 marks (20 each) will be awarded for correct observation, use of correct formula, calculation and result within permissible limits as follows:

  • Theory and procedure                                                                     5 marks
  • Correct observation and proper recording                                      5 marks
  • Use of correct formula and calculation                                           5 marks
  • Result                                                                                              5 marks

Viva:

Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understood the basic principles of chemistry.

Academic Year: 
2022-2023
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