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Chemistry Laboratory I
Course Objectives:
This course will enable the students to –
be acquainted with various safety measures, detection of extra elements, chromatographic techniques and use of apparatus used in volumetric analysis with correct titrimetric procedure.
Course Outcomes (COs):
|
Course Outcomes |
Teaching Learning Strategies |
Assessment Strategies |
|
|---|---|---|---|
On completion of this course, the students will be able to –
CO6: apply the knowledge of lab safety during the experimental work. CO7: develop the analytical skills to carry out different types of volumetric analysis. CO8: determine the presence of extra elements in the given organic compound CO9: apply chromatographic technique to separate and identify different components of mixture. |
• Laboratory instructions and Demonstrations |
· • Conduction of Experiments
|
Laboratory Safety measures
Handling of hazardous chemicals, incompatible chemicals, flammable solvents, fire hazards in chemical laboratory, control of fire, fire extinguishers, toxicity of chemicals, forms of toxic materials, personal protective equipment, health effects and first aid, MSDS (material safety data sheet), use of compressed gases, waste minimization strategies and chemical waste disposal, procedures for neutralization of strong acids and strong bases.
Volumetric Analysis
Theoretical aspects-classification and primary requirements of titrations.
Acid-base titrations- basic principle, neutralisation indicators –types, theories of indicators, choice of indicators, titration curves and applications.
Redox titrations-principle, indicators, and applications of permangnatometry, dichromatometry, iodometry titrations.
Complexometric titration-reactions and properties of EDTA, types of EDTA titrations.
Calibration of fractional weights, pipettes and burettes, preparation of standard solutions, dilution – 0.1 M to 0.001 M solutions, buffer solutions.
1. Estimation of sodium carbonate and sodium hydrogen carbonate present in a mixture.
2. Estimation of oxalic acid by titrating it with KMnO4.
3. Estimation of water of crystallization in Mohr’s salt by titrating with KMnO4.
4. Estimation of Fe (II) ions by titrating it with K2Cr2O7 using internal indicator.
5. Estimation of Cu (II) ions iodometrically using Na2S2O3
6. Estimation of total hardness of water sample
Organic Chemistry Experiments
1. Detection of extra elements (N, S, Cl, Br, I) in organic compounds (containing upto two extra elements)
2. Separation of mixtures by chromatography: Measure the Rf value in each case
(combination of two compounds to be given)
a. Identify and separate the components of a given mixture of 2 amino acids (glycine,aspartic acid, glutamic acid, tyrosine or any other amino acid) by paper chromatography
b. Identify and separate the sugars present in the given mixture by paper chromatography
• Advanced University Practical Chemistry, Part I, First Edition; P.C. Kamboj, Vishal Publishing House, New Delhi; 2016.
SUGGESTED READINGS:
• Vogel’s Textbook of Quantitative Chemical Analysis; Sixth Edition; M. Thomas, B. Sivasankar, J. Mendham, R.C. Denney, J. D. Barnes; Pearson Education, New Delhi, 2009
• Textbook of Practical Organic Chemistry, Fifth edition; Vogel, A.I., Tatchell, A.R., Furnis, B.S., Hannaford, A.J. & Smith, P.W.G., Prentice-Hall, 1996.
e-RESOURCES:
• https://vlab.amrita.edu/index.php?sub=2&brch=191&sim=344&cnt=1 (Element Detection)
• https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Supplementa...(Analytical_Chemistry)/Quantifying_Nature/Volumetric_Chemical_Analysis_(Shiundu) (Volumetric Analysis)
• https://www.selfstudys.com/books/cbse-lab-manual/english/12th/11-volumet... (Volumetric Analysis)
• https://egyankosh.ac.in/simple-search?query=titration (Volumetric Analysis)
• https://egyankosh.ac.in/bitstream/123456789/43262/1/Unit-5.pdf (Lab Safety)
Scheme of Examination
The duration of examination will be 4 hrs.
The following exercises will be set in the examination –
Quiz based on lab safety measures 10 marks
Volumetric Analysis 30 marks
Organic Chemistry 18 marks
Viva 12 marks
Total 70 marks
1 Quiz based on Lab Safety Measures
The Quiz will consist of 10 objective type questions (Multiple Choice Questions/Fill in the blanks/One-word answer/True or False/Match the following etc.) carrying 1 mark each. Duration of the quiz will be 10 minutes.
2 Volumetric Analysis
The exercise carries 30 marks. The distribution of marks will be as follows:
Six marks will be reserved for the preparation of solutions, neat methodical and systematic working of the candidate.
The remaining 24 marks will be distributed as follows:
- Theory 4 marks
- Correct observation and proper recording 6 marks
- Use of correct formula and calculation 6 marks
- Result 8 marks
|
% Error |
Marks awarded |
|---|---|
|
≤1.0 |
8 |
|
>1.0 & <1.5 |
6 |
|
>1.5 & <2 |
4 |
|
>2 |
0 |
3. Organic Chemistry Experiments
- Detection of extra elements 8 marks
- Chromatographic Techniques:
One chromatographic separation of 10 marks will be given. The distribution of marks is as follows:
- Theory and Procedure 2 marks
- Preparation of solutions 3 marks
- Observation 3 marks
- Result 2 marks
4. Viva
Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understood the basic principles.
