Chemistry Laboratory IX

Paper Code: 
24CCHY513
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to

understand the handling of UV-vis spectrophotometer and its use in various applications.

 

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and Teaching Strategies

Assessment Strategies

Course Code

Course

title

 

24CCHY513

 

Chemistry Laboratory IX

 

CO101: Apply spectrophotometric techniques to record absorbance spectra and analyze it .

CO102: Estimate the concentration of a given sample using Lambert-Beer’s law.

CO103: Determine the dissociation constant and interpret the kinetics of a reaction using

Spectrophotometer.

CO104: Perform scientific experiments as well as accurately record experimental data and analyze the results of such experiments.

CO105: Compile and report the results efficiently, and develop communications skills.

CO106: Contribute effectively in course-specific interaction.

Approach in teaching:

Power point presentation, laboratory instructions and demonstrations.

 

 

Learning activities for the students:

Peer learning, hands-on-learning, interpretation of data, record

writing and learning through virtual labs.

 

Conduction of experiments, lab records, viva voce.

 

 

 

Unit I: 

Minor Experiments

  • Study the absorbance spectra of KMnO4 and K2Cr2O7 (in 0.1 M H2SO4) and determine the λmax values. Calculate the energies of the two transitions indifferent units (J molecule-1, kJ mol-1, cm-1, eV).
  • Study the pH-dependence of the UV-Vis spectrum of K2Cr2O7.
  • Record the 200-350 nm UV spectra of the given compounds (acetone, acetaldehyde, 2-propanol, acetic acid) in water. Comment on the effect of structure on the UV spectra of organic compounds.
  • Analyse the given vibrational-rotational spectrum of HCl.

 

Major Experiments

  • Verify Lambert-Beer’s law and determine the concentration of CuSO4/KMnO4/K2Cr2O7 in a solution of unknown concentration.
  • Determine the concentrations of KMnO4 and K2Cr2O7 in a mixture spectrophotometrically.
  • Study the kinetics of iodination of propanone in acidic medium. (volumetrically/spectrophotometrically)
  • Determine the amount of iron present in a sample using 1,10-phenathroline spectrophotometrically.
  • Determine the dissociation constant of an indicator phenolphthalein spectrophotometrically.
  • Study the kinetics of interaction of crystal violet/ phenolphthalein with sodium hydroxide. (volumetrically/spectrophotometrically)

 

Scheme of Examination

 

The duration of examination will be 5 hrs.

The following exercises will be set in the examination

Minor experiment                                                                       20 marks

Major experiment                                                                       35 marks

Viva                                                                                          15 marks

Total                                                                         70 marks

 

The candidates shall be given two exercise out of exercises prescribed in the syllabus. The exercises in physical chemistry can be modified by the examiner and the same will be explained to the candidates. However, such modifications will not involve any departure from the basic principles over which experiments are prescribed in the syllabus.

The distribution of marks will be as follows

                                                            Minor                            Major

Theory and procedure                           2 marks                        5 marks

Correct observation                               6 marks                        12 marks

Use of correct formula                           2  marks                        5 marks

Calculation/Interpretation                       6  marks                        8 marks

Results                                                  4  marks                        5 marks

 

Viva

Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understanding the basic principles.

 

Essential Readings: 
  1. Advanced Practical Physical Chemistry, Thirtieth Edition; J. B. Yadav; Krishna Prakashan Media Pvt. Ltd., Meerut, 2015.

 

References: 
  1. Experimental Physical Chemistry, First Edition; V. D. Athawale and P. Mathur; New age International (P) Ltd. Publishers, New Delhi, 2011.
  2. Experiments in Physical Chemistry, Fifth Edition; D. P. Shoemaker, C. W. Garland and J. W. Nibler, Mc Graw-Hill, New York, 1998.

e-Resources:

  1. https://vlab.amrita.edu/?sub=2&brch=190&sim=338&cnt=1 (spectrophotometry)
  2. http://mas-iiith.vlabs.ac.in/List%20of%20experiments.html (kinetics, pH effect, verify Beer-Lambert’s law)

 

Academic Year: