Chemistry Laboratory V (B)

Paper Code: 
24DCHY502(B)
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to

understand the principles and applications of various separation techniques, including chromatography, solvent extraction and ion exchange, apply them to separate and identify compounds in different types of mixtures, perform various analytical techniques, including spectrophotometry, flame photometry and ion exchange chromatography to determine the properties of different samples, such as pH and metal ion concentrations.

 

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and Teaching Strategies

Assessment Strategies

Course Code

Course

Title

 

DCHY 502(B)

 

 

 

Chemistry Laboratory V (B)

(Practical)

 

CO66: Perform and interpret the results of various separation techniques, including paper chromatography, TLC, ion exchange chromatography and solvent extraction methods, and apply them to separate and identify compounds in mixtures.

CO67: Operate various analytical instruments, including spectrophotometry, flame photometry, to determine the pH, nutrient content, and other parameters of various samples, and interpret the results to draw conclusions about the samples' properties.

CO68: Identify and apply appropriate separation techniques and analytical methods for different types of samples, such as soil, aerated drinks, fruit juices, and shampoos.

CO69: Compile and report the results efficiently, and develop communications skills.

CO70: Contribute effectively in course-specific interaction

Approach in teaching:

Laboratory instructions and

demonstrations.

 

Learning activities for the students:

Peer learning, hands-on-learning,

interpretation of data, record writing and learning through virtual labs.

 

Conduction of experiments, lab records, viva voce and quiz.

 

 

Unit I: 

Major Experiment

  1. To separate a mixture of Ni2+ & Fe2+ by complexation with DMG and extracting the Ni2+- DMG complex in chloroform and determine its concentration by spectrophotometry.
  2. Solvent extraction of zirconium with amberliti LA-1, separation from a mixture of iron and gallium.
  3. Determination of Na, Ca, Li in cola drinks and fruit juices using flame photometric techniques.
  4. Estimation of calcium, magnesium, phosphate, nitrate in soil sample.
  5. Determination of pKa values of indicator using spectrophotometry.
  6. Determine the composition of the Ferric-salicylate/ ferric-thiocyanate complex by Job’s method.
  7. Determination of exchange capacity of cation exchange resins and anion exchange resins.
  8. Separation of metal ions from their binary mixture using ion exchange chromatography.
  9. Separation of amino acids from organic acids by ion exchange chromatography.
  10. To determine conductometrically the second order rate constant for the hydrolysis of ethyl acetate by sodium hydroxide.

 

Minor Experiment

  1. Determination of pH of soil sample and estimate total soluble salt in this soil sample.
  2. To determine the specific conductivity of soil sample.
  3. Record an absorption spectrum of a substance (KMnO4/K2Cr2O7) using a spectrophotometer and determine absorption maxima.
  4. To verify the Beer-Lambert law for a compound (potassium permanganate, copper sulphate, methylene blue etc.) and determine the concentration of the substance using calibration curve.
  5. Chromatographic separation (TLC) and identification on the basis of Rf values of -
  1. Mixture of Sudan Yellow and Sudan Red (Azo dyes)
  2. Active ingredients of plants, flowers, fruit juices  and identify them on the basis of their Rf values.
  3. Mixture of organic compounds

 

Demonstrations

  1.  Determination of dissolved oxygen in water.
  2.  Determination of chemical oxygen demand (COD).
  3.  Determination of Biological oxygen demand (BOD).

 

Scheme of Examination

The duration of examination will be 4 hrs.

The following exercises will be set in the examination

Major Experiment                                                        38 marks

Minor Experiment                                                        20 marks

Viva                                                                           12 marks

            Total                                                                              70 marks

1.Analytical Chemistry Experiments

The students will be allotted two experiments to perform in the lab. The exercises can be modified by the examiners and the same will be explained to the candidates. However, such modifications

 will not involve any departure from the basic principles over which experiments are prescribed in the syllabus.

Candidates shall be given one major and one minor exercise out of the exercises prescribed in the syllabus. The distribution of marks will be as follows:

                                                                      Major                          Minor

  • Theory and procedure                              8 marks                          4 marks
  • Correct observation                                  8 marks                          5 marks
  • Use of correct formula                              8 marks                          4 marks
  • Calculation                                                8 marks                          5 marks
  • Result                                                        6 marks                          2 marks

 

No marks for calculation will be awarded to a candidate for not calculating result.

The permissible error will depend on the nature of the experiment. One mark shall be deducted for each + 2% error over permissible limit.

 

2.Viva

Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understanding the basic principles.

 

Essential Readings: 
  1. Advanced Practical Physical Chemistry, Thirtieth Edition; J. B. Yadav; Krishna Prakashan Media Pvt. Ltd., Meerut, 2015.
  2. Advanced University Practical Chemistry, Part I, First Edition; P.C. Kamboj, Vishal Publishing House, New Delhi; 2016.

 

References: 
  1. Instrumental Methods of Analysis, Seventh Edition; Willard, Hobert H. et. Al; CBS; 2004.
  2. Laboratory Hand Book of Chromatographic & Allied Methods, Mikes O. & Chalmes, R.A; Halsted Press; 1979.
  3. Analytical Chemistry-Methods of Separation, Ditts R.V.; Van Nostrand; 1974.

 

 

e-Resources:

  1. https://vlab.amrita.edu/index.php?sub=2&brch=193&sim=1315&cnt=1 (soil conductivity)
  2. https://vlab.amrita.edu/index.php?sub=2&brch=193&sim=1549&cnt=1 (soil pH)
  3. https://vlab.amrita.edu/?sub=2&brch=190&sim=338&cnt=1 (spectrophotometry)
  4. https://www.academia.edu/34558723/EXPERIMENT_3_KINETICS_OF_A_SECOND_ORDER_REACTION (kinetics)
  5. https://nptel.ac.in/courses/104106121(chromatography)

 

Academic Year: 
2024-2025
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