CHEMISTRY LABORATORY XI A

Paper Code: 
D-CHY 516 A
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objective(s):
This course will enable the students to–

learn and analyze proteins, amino acids, fats and oils quantitatively.

 

Course Outcomes (COs):

Course Outcomes

Teaching Learning

Strategies

Assessment

Strategies

On completion of this course, the students will be able to-

CO92: estimate the amount of proteins, amino acids and saponification value of oils and fats.

CO93: isolate and characterize DNA from onion/ cauliflower/peas.
  • Laboratory

instructions and demonstrations

 
  • Conduction of experiments
  • Lab records
  • Viva voce

 

 

60.00
Unit I: 

Experiments:

  • Estimation of glycine by Sorenson’s formalin method.
  • Study of the titration curve of glycine.
  • Estimation of proteins by Lowry’s method.
  • Study of the action of salivary amylase on starch at optimum conditions.
  • Effect of temperature on the action of salivary amylase.
  • Saponification value of an oil or a fat.
  • Determination of Iodine number of an oil/ fat.
  • Isolation and characterization of DNA from onion/ cauliflower/peas.

Any other experiment related to prescribed topic can be carried out in the class.

 

Essential Readings: 
  • Manual of Biochemistry Workshop, Department of Chemistry, University of Delhi, 2012.
  • Textbook of Practical Organic Chemistry, 5th edition, A.I. Vogel,A.R. Tatchell, B.S.Furnis, A.J. Hannaford & P.W.G. Smith, Prentice-Hall, 1996.

 

References: 

SUGGESTED READINGS:

  • Comprehensive Practical Organic Chemistry, Ahluwalia, V.K. & Aggarwal, R., Universities Press, 2001.

e-RESOURCES:

Scheme of Examination

The duration of examination will be 5 hrs.
The following exercises will be set in the examination
Organic Chemistry Experiments (two)

Experiment I                                                                  27 marks
Experiment II                                                                 28 marks
Viva                                                                               15 marks
Total                                                                                 70 marks

The exercise carries 27 marks / 28 marks. The exercises in Organic chemistry can be modified by the examiners and the same will be explained to the candidates. However, such modifications will not involve any departure from the basic principles over which experiments are prescribed in the syllabus.
Candidates shall be given two exercise out of the exercises prescribed in the syllabus. Four marks shall be awarded for preparatory work, setting up of an apparatus, neat and methodical work.
Twenty three / Twenty four marks will be awarded for theory and procedure, correct observation and proper recording, use of correct formula, calculation and result within permissible limits. The distribution of marks will be as follows:

Theory and procedure                                                   6 marks
Correct observation and proper recording                       5 marks
Use of correct formula and calculation                            6 marks
Result                                                                      6 marks / 7 marks

The permissible limit will be decided by the examiner as it varies with the nature of the experiment.

Viva

Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understood the basic principles.

 

 

Academic Year: 
2023-2024
  • Printer-friendly version
  • PDF version