Coming soon
Chemistry Laboratory XI(A)
This course will enable the students to
learn and analyze proteins, amino acids, fats and oils quantitatively.
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Course |
Learning Outcome (at course level) |
Learning and Teaching Strategies |
Assessment Strategies |
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Course Code |
Course title |
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24DCHY516(A)
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Chemistry Laboratory XI(A) (Practical)
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CO118: Estimate the amount of proteins, amino acids and saponification value of oils and fats. CO119: Isolate and characterize DNA from onion/ cauliflower/peas. CO120: Compile and report the results efficiently, and develop communications skills. CO121: Contribute effectively in course-specific interaction.. |
Approach in teaching: Power point presentation, laboratory instructions and demonstrations.
Learning activities for the students: Peer learning, hands-on-learning, interpretation of data, record writing and learning through virtual labs.
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Conduction of experiments, lab records, viva voce.
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Experiments
- Estimation of glycine by Sorenson’s formalin method.
- Study of the titration curve of glycine.
- Estimation of proteins by Lowry’s method.
- Study of the action of salivary amylase on starch at optimum conditions.
- Effect of temperature on the action of salivary amylase.
- Saponification value of an oil or a fat.
- Determination of Iodine number of an oil/ fat.
- Isolation and characterization of DNA from onion/ cauliflower/peas.
Any other experiment related to prescribed topic can be carried out in the class.
Scheme of Examination
The duration of examination will be 5 hrs.
The following exercises will be set in the examination
Organic Chemistry Experiments (two)
Experiment I 27 marks
Experiment II 28 marks
Viva 15 marks
Total 70 marks
The exercise carries 27 marks / 28 marks. The exercises in Organic chemistry can be modified by the examiners and the same will be explained to the candidates. However, such modifications will not involve any departure from the basic principles over which experiments are prescribed in the syllabus.
Candidates shall be given two exercise out of the exercises prescribed in the syllabus. 4 marks shall be awarded for preparatory work, setting up of an apparatus, neat and methodical work.
Twenty three / Twenty four marks will be awarded for theory and procedure, correct observation and proper recording, use of correct formula, calculation and result within permissible limits. The distribution of marks will be as follows:
Theory and procedure 6 marks
Correct observation and proper recording 5 marks
Use of correct formula and calculation 6 marks
Result 6 marks / 7 marks
The permissible limit will be decided by the examiner as it varies with the nature of the experiment.
Viva
Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understood the basic principles.
- Manual of Biochemistry Workshop, Department of Chemistry, University of Delhi, 2012.
- Textbook of Practical Organic Chemistry, 5th edition, A.I. Vogel, A.R. Tatchell, B.S.Furnis, A.J. Hannaford & P.W.G. Smith, Prentice-Hall, 1996.
- Comprehensive Practical Organic Chemistry, Ahluwalia, V.K. & Aggarwal, R., Universities Press, 2001.
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