Chemistry Laboratory XIV(B)

Paper Code: 
24DCHY616(B)
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to

apply the fundamental concepts/theory of different instrumental methods and analytical techniques used in chemistry.

 

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and Teaching Strategies

Assessment Strategies

Course Code

Course

title

 

24DCHY616(B)

 

 

 

Chemistry Laboratory XIV(B)

(Practical)

 

CO172:  Perform the experiments based on UV/Vis spectrophotometry, pH metry and atomic absorption spectrometry.

CO173: Interpret IR, NMR spectra of various organic compounds.

CO174: Apply chromatographic technique for separation of organic compounds.

CO175: Perform scientific experiments as well as accurately record experimental data and analyze the results of such experiments and develop communication skills.

CO176: Contribute effectively in course-specific interaction.

Approach in teaching:

Power point presentation, laboratory instructions and demonstrations.

 

 

Learning activities for the students:

Peer learning, hands-on-learning, interpretation of data, record

writing and learning through virtual labs.

 

Conduction of experiments, lab records, viva voce.

 

 

 

Unit I: 

A list of experiments given below, students are required to perform 8-10 experiments:

  • To titrate Fe(II) with KMnO4 spectrophotometrically.
  • To determine the concentration of Fe (II) by spectrophotometric titration with EDTA.
  • To titrate copper solution with EDTA spectrophotometrically.
  • Detection of adulteration of food stuffs: To detect the total dye content in sunset yellow colour.
  • Determination of a mixture of cobalt and nickel (UV/Vis spec.).
  • Study of electronic transitions in organic molecules (i.e., acetone in water and related system)
  • Determination of vitamin C in fruit. (UV/Vis spec.).
  • Determination of the isoelectric pH of a protein.
  • Titration curve of an amino acid.
  • Interpretation of IR and NMR spectra of simple organic compounds (ethanol, 1-propanol, 2-propanol, methylethanoate, ethyl acetate, stilbene, ethyl acetate, propanone etc).
  • IR Absorption Spectra (study of aldehydes and ketones in different solvents).
  • Determination of calcium, iron, and copper in food by atomic absorption.
  • Separation of carbohydrates by HPLC/paper chromatography.
  • Determination of caffeine in beverages by HPLC.
  • Potentiometric titration of a chloride-iodide mixture.
  • Cyclic voltammetry of the ferrocyanide/ferricyanide couple.
  • Determination of the void volume of a gel filtration column.
  • Use of fluorescence to do “presumptive tests” to identify blood or other body fluids.
  • Use of “presumptive tests” for anthrax or cocaine.
  • Use of capillary electrophoresis with laser fluorescence detection for nuclear DNA (Y chromosome only or multiple chromosome).
  • Quantitative analysis of mixtures by gas chromatography (i.e., chloroform and carbon tetrachloride).
  • Laboratory analysis to confirm anthrax or cocaine.
  • Detection in the field and confirmation in the laboratory of flammable accelerants or explosives.
  • Detection of illegal drugs or steroids in athletes.
  • Detection of pollutants or illegal dumping.
  • Fibre analysis.

Any other experiment related to topic carried out in the class.

 

Scheme of Examination

 

The duration of examination will be 5 hrs.

The following exercises will be set in the examination:

Physical experiment                                                                   58 marks

Viva                                                                                          12 marks

Total                                                                                        70 marks       

 

The exercise carries 58 marks. The candidates shall be given either one exercise of 58 marks or two exercise of 29 marks each out of exercises prescribed in the syllabus. The exercises in physical chemistry can be modified by the examiner and the same will be explained to the candidates. However, such modifications will not involve any departure from the basic principles over which experiments are prescribed in the syllabus.

The distribution of marks will be as follows

Theory and procedure                                                               8 marks

Correct observation                                                                   18 marks

Use of correct formula                                                               8 marks

Calculation                                                                                16 marks

Result                                                                                       8 marks

 

Viva

Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understanding the basic principles.

 

Essential Readings: 
  1. Advanced Practical Physical Chemistry, Eighteenth Edition; J.B. Yadav; Goel Publishing House, Meerut, 2015.
  2. Principles of Instrumental Analysis, Seventh Edition; Skoog, D.A., Holler F.J. and S.R. Crouch; Cengage Learning, USA, 2016.

 

References: 
  1. Experimental Physical Chemistry; First Edition; V. D. Athawale, P. Mathur; New Age International (P) Ltd. Publishers, New Delhi, 2011.
  2. Advanced University Practical Chemistry, Part I, First Edition; P.C. Kamboj, Vishal Publishing House, New Delhi; 2013.

e-Resources:

  1. https://egyankosh.ac.in/bitstream/123456789/79539/1/Unit-5.pdf (Job’s method)
  2. https://vlab.amrita.edu/?sub=3&brch=63&sim=1336&cnt=1#:~:text=Titration%20curves%20are%20obtained%20when,per%20mole%20of%20the%20sample. (Titration curve of amino acid)
  3. https://vlab.amrita.edu/?sub=2&brch=190&sim=338&cnt=1 (UV)

 

 

Academic Year: 
2024-2025
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