Coming soon
Laboratory Course
Paper Code:
BCH 103
Credits:
2
Contact Hours:
60.00
Max. Marks:
100.00
Objective:
1. Introduction to concentration scales- Definition , units of various concentration terms.
2. Preparation of solutions and their standardization.
3. Preparation of standard buffers and determination of pH of a solution.
4. Verification of Beer Lambert’s Law.
5. Qualitative tests for :
- Carbohydrates
- Proteins and amino acids
- Lipids
6. Determination of saponification value and iodine number of fats.
7. Estimation of ascorbic acid by titrimetric method using 2,6-dichlorophenol indophenol.
8. Estimation of
- Carbohydrate by anthrone method/Benedicts method.
- Blood glucose by the methods
a) Folin-Wu
b) Nelson-Somogyi
9. Isolation and assay of glycogen from rat liver.
10. Extraction of total lipids by Folch method
Unit I:
References:
- Vogel’s Textbook of Quantitative Chemical Analysis, Fifth Edition, G.H. Jeffery, J. Bassett. J. Mendham, R.C. Denney, Longman Scientific and Technical Publication, England, 1991.
- An Introduction to Practical Biochemistry, Third edition, Plummer, Tata Mc Graw Hill, 2002.
Academic Year:
