Laboratory Course
Objective:
- Separation of sugars/ amino acids using paper chromatography/TLC.
- Titration curve for amino acids and determination of pK value.
- Estimation of amino acids by ninhydrin method.
- Colorimetric/ Spectrophotometric estimation of amino acids.
- Separation and detection/identification of amino acids by electrophoresis.
References:
- An Introduction to Practical Biochemistry, Third edition, Plummer, Tata Mc Graw Hill, 2002.
- Vogel’s Text Book of Practical Organic Chemistry, Fifth Edition, B.S. Furniss, A.J. Hannaford, P.W.G. Smith, A.R. Tatchell; Adission – Wesley Longman Ltd. England, 1998.
- Hawk’s Physiological Chemistry, Bernard L Oser, Tata Mc Graw Hill, 1954.