Coming soon
Laboratory Course VIII
Course Objectives:
This course will enable the students to -
- make the students understand the concept of quatitative gravimetric analysis method.
- provide an in-depth knowledge of various chromatographic techniques.
Course Outcomes (COs):
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Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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CHY-416 |
Laboratory Course VIII
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The students will be able to –
CO121: apply quantitative gravimetric analysis method. CO122: estimate percentage and number of functional groups by quantitative analysis method. CO123: separate green leaf pigments by TLC and ascending paper chromatography. CO124: apply circular paper chromatography and column chromatography. |
Interactive Lectures
Discussions
Tutorials
Substantial laboratory-based practical component and experiments
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Lab Tests and viva voce at Periodic Intervals.
Semester End Examination
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Quantitative Analysis: Gravimetric Analysis (Any Three)
a) Estimation of Cu as CuSCN,
b) Estimation of Zn as Zinc ammonium phosphate,
c) Estimation of Pb as PbCrO4
d) Estimation of Ni as Ni(DMG)2
Quantitative Analysis: Estimation of functional groups
a) Determination of the percentage and number of hydroxyl groups in an organic compounds by acetylation method.
b) Estimation of amines/phenols using bromated/bromide solution.
Chromatography
a) Separation of 2,4 –dinitrophenylhydrazones of acetone, 2-butanone, hexan-2- one and hexane-3-one using toluene and light petroleum (40:60) by TLC
b) Separation of green leaf pigments (spinach leaves may be used) by TLC
c) Separation of green leaf pigments (spinach leaves may be used) by ascending paper chromatography
d) Separation of amino acids or carbohydrates by circular paper chromatography.
e) Separation of mixture of organic compounds by column chromatography.
- Vogel’s Textbook of Quantitative Chemical Analysis; Sixth Edition; M. Thomas, B. Sivasankar, J. Mendham, R.C. Denney, J. D. Barnes; Pearson Education, New Delhi, 2009.
- Vogel’s Textbook of Practical Organic Chemistry; Fifth Edition; B.S. Furniss, A.J. Hannaford, P.W.D. Smith, A.R. Tatchell; Pearson Education, New Delhi, 2003.
