Laboratory course XI

Physical Chemistry Experiments

Colorimetry

a) Job’s method of continuous variation by iron-phenanthroline complex.

b) Mole Ratio method by iron-phenanthroline complex.

c) Record an absorption spectrum of a substance (KMnO₄/K₂Cr₂O₇) using a spectrophotometer and determine absorption maxima (λ_max).

d) To verify the Beer-Lambert law for a compound (Potassium permanganate, copper sulphate, methylene blue etc.) and determine the concentration of the substance using calibration curve.

Polarimeter
e) Determine the rate constant of the inversion of cane sugar in presence of hydrochloric acid and sulphuric acid by using polarimeter and evaluate the relative strength of the two acids.

Spectrophotometric Estimation

i. Protein

ii. Carbohydrate

iii. Cholesterol