Laboratory Course XII

Physical Chemistry Experiments

Colorimetry

1. Job’s method of continuous variation by iron-phenanthroline complex.
2. Mole Ratio method by iron-phenanthroline complex.
3. Record an absorption spectrum of a substance (KMnO4/K2Cr2O7) using a spectrophotometer and determine absorption maxima (λmax).
4. To verify the Beer-Lambert law for a compound(Potassium permanganate, copper sulphate,methylene blue etc.) and determine the concentration of the substance using calibration curve.

Polarimeter

1. Determine the rate constant of the inversion of cane sugar in presence of hydrochloric acid and sulphuric acid by using polarimeter and evaluate the relative strength of the two acids.

Spectrophotometric Estimations

1. Protein
2. Carbohydrate
3. Cholesterol

Marking Instructions

1. The duration of examination will be 4 hrs.
2. The following exercises will be set in the examinations:

1. PhysicalExperiment (two major experiment)                         27+28 marks
2. Viva                                                                                        15 marks

Total 70 marks

3. Physical Chemistry Experiment:

The exercise carries 55 marks. Two major experiments of 27 and 28 marks respectively will be given to candidate by lot. Fifteen marks (7 and 8) shall be awarded for preparatory work, setting up of an apparatus, neat and methodical work.

40 marks (20 each) will be awarded for correct observation, use of correct formula, calculation and result within permissible limits as follows:

1. Theory and procedure                                             5 marks
2. Correct observation and proper recording                  5 marks
3. Use of correct formula and calculation                      5 marks
4. Result                                                                   5 marks

4. Viva:

Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understanding the basic principles of chemistry.