Laboratory course XII

Paper Code: 
CHY-616
Credits: 
2
Contact Hours: 
60.00
Objective: 

Course Objectives:

This course will enable the students to –

  1. get an insight on the use of apparatus used in colorimetry like spectrophotometer and to enable them to record absorption spectrum of substances.

  2. acquire knowledge of spectrophotometric estimations of proteins, carbohydrates and cholesterols

 

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

CHY 616

 

 

 

Laboratory course XI           

 

 

The students will be able to –

 

CO178: apply Beer’s Law, relating solution concentration to absorbance and use the measurement of light absorption (colorimetry) to detect the colorimetric reaction of thiocyanate with iron

CO179:  perform the spectrophotometric estimations of protein, carbohydrate and cholesterol along with their calculations

CO180: interpret the method of continuous variation to determine the reaction stoichiometry for the formation of a metal ion complex

CO181: learn the technique of absorption spectroscopy in order to monitor the relative concentration of a colored metal ion complex in solution
  • Class Lecture
  • Discussion
  • Demonstration
  • Substantial laboratory-based practical component and experiments

 
  • Continuous Assessment (practical test)
  • Viva voce
  • Semester end examinations and observation

 

Unit I: 

Physical Chemistry Experiments

Colorimetry

a) Job’s method of continuous variation by iron-phenanthroline complex.

b) Mole Ratio method by iron-phenanthroline complex.

c) Record an absorption spectrum of a substance (KMnO4/K2Cr2O7) using a spectrophotometer and determine absorption maxima (λmax).

d) To verify the Beer-Lambert law for a compound(Potassium permanganate, copper sulphate, methylene blue etc.) and determine the concentration of the substance using calibration curve.

Polarimeter
e) Determine the rate constant of the inversion of cane sugar in presence of hydrochloric acid and sulphuric acid by using polarimeter and evaluate the relative strength of the two acids.

Spectrophotometric Estimations

  1. Protein
  2. Carbohydrate
  3. Cholesterol

 

References: 
  • Vogel’s Textbook of Practical Organic Chemistry; Fifth Edition; B.S. Furniss, A.J. Hannaford, P.W.D. Smith, A.R. Tatchell; Pearson Education, New Delhi, 2003.
  • Advanced Practical Physical Chemistry; Eighteenth Edition; J.B. Yadav; Goel Publishing House, Meerut, 2015.
Academic Year: 
2021-2022
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