Organic Chemistry Lab II

Paper Code: 
24CHY327
Credits: 
3
Contact Hours: 
90.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to

acquire knowledge of synthesis by conventional and non-conventional methods, handling of hazardous chemicals and to enhance learning of physical and spectroscopic techniques.

 

Course Outcomes: 

Course

Learning outcome

(at course level)

Learning and Teaching Strategies

Assessment Strategies

Course Code

Course

Title

 

24CHY327

 

Organic Chemistry Lab II

(Practical)

CO128:  Characterize organic molecules by different spectral techniques.

CO129: Demonstrate and apply common laboratory techniques for organic synthesis.

CO130:Employ spectrophotometric techniques for the estimations of prescribed biomolecules.

CO131:Contribute effectively in course-specific interaction.

Approach in teaching:

Laboratory instructions and

Demonstrations.

 

Learning activities for the students:

Peer learning, hands-on-learning,

interpretation of data, record writing and learning through virtual labs

 

  • Conduction of Experiments, Lab Records, Viva Voce, Quiz

 

Unit I: 

Green Chemistry                                                                             (10 Hours)

Green Chemistry & sustainability, twelve principles of green chemistry, atom economy, E-factor, green chemistry tools.

Solvents:Types based on hazards, applications, green solvents-supercritical fluids, ionic liquids, water, solvent free solid phase reaction.

Alternative energy sources, ultrasound, microwave, reactions at high pressure in constrained media, renewable sources, C1 chemistry.

Green catalysts-types, advantages and disadvantages, applications in organic synthesis.

Synthesis (Green Methods / Conventional Methods)                     (30 Hours)

  1. One step synthesis
    • Aldol condensation (Synthesis of dibenzal propanone)
    • Acetylation (synthesis of acetanilide from aniline)
    • Pechmann Condensation for Coumarin synthesis (Clay catalyzed solid state synthesis of 7- hydroxy -4-methyl coumarin)
    • Bromination of phenol

 

  1. Two step synthesis (any three)
    • Benzoin → benzil → benzilic acid
    • Benzophenone → benzopinacol → benzopinacolone
    • Acetanilide → p-bromoacetanilide → p-bromoaniline
    • Acetanilide → p-nitroacetanilide → p-nitroaniline
    • Resorcinol → fluorescein → eosin
    • Phthalic anhydride → phthalimide → anthranilic acid

 

Extraction of Organic Compounds from Natural Resources (any three)                                                                                                              (20 Hours)

  • Isolation of caffeine from tea leaves.
  • Isolation of casein from milk.
  • Isolation of lactose from milk.
  • Isolation of nicotine dipicrate from tobacco.

 

Spectroscopy                                                                                 (10 Hours)

Identification of organic compounds (with emphasis on isomeric studies and calculation of % ratio of tautomers) by the spectral analysis.

Spectrophotometric Estimations (any three)                           (20 Hours)

  • Protein
  • Carbohydrate
  • Cholesterol
  • Phosphate
  • Ascorbic acid

 

Scheme of Examination

 

The duration of examination will be 6 hrs.

The following exercises will be set in the examination

Organic Synthesis                                                          26 marks

Spectral Problems                                                          14 marks

Spectrophotometric Estimation                                      15 marks

Viva                                                                             15 marks

Total                                                                            70 marks

 

1.Organic synthesis

This exercise carries 26 marks. Either 2 one step synthesis or two step synthesis will be allotted to the candidates by lot. The yield, purity, crystallization and recording of m.p. will be given due consideration while awarding marks. 

 

2.Spectral Problems

This exercise carries 14 marks. The students may be provided with either spectral problems or data base problems.

 

3.Spectrophotometric Estimation

This exercise carries 15 marks. Three marks are reserved for the preparatory work, setting up of an instrument and experimental skills. The distribution of remaining 12 marks are as follows.

Correct observation and proper recording                                   4 marks

Standard curve                                                                          4 marks

Result                                                                                       4marks

 

4.Viva

Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understanding the basic principles of chemistry.

 

Essential Readings: 
  1. Vogel’s Textbook of Practical Organic Chemistry, Fifth Edition, B.S. Furniss, A.J. Hannaford, P.W.G. Smith, A.R. Tatchell; Addison – Wesley Publishing Company, 2014.
  2. Practical Organic Chemistry, Fourth Edition; F.C. Mann and B.C. Saunders; Pearson Education, New Delhi, 2013.

 

References: 
  1. Advanced Practical Organic Chemistry, J. Leonard, B. Lygo and G. Procter; Third Edition; CRC Press, 2013.
  2. Green Chemistry: Introductory Text, Third Edition; M. Lancaster; Royal Society of Chemistry (London), 2016.

 

e-Resources:

  1. https://chem.libretexts.org/Bookshelves/Ancillary_Materials/Laboratory_Experiments/Wet_Lab_Experiments/Organic_Chemistry_Labs/Experiments/3%3A_Extraction_of_Caffeine_(Experiment) (Isolation of caffeine from tea leaves)
  2. https://youtu.be/sPhJWBL17OQ (Isolation of caffeine from tea leaves)
  3. https://vlab.amrita.edu/?sub=3&brch=63&sim=158&cnt=1 (Isolation of casein from milk-
  4. https://studylib.net/doc/5885894/experiment-5--isolation-of-casein-and-lactose-from-milk (Isolation of lactose from milk)
  5. https://youtu.be/DBS5g4BoSZY  (Isolation of lactose from milk) https://youtu.be/16lGxX19x9M (Isolation of nicotine dipicrate from tobacco)
  6. http://www.ijcrt.org/papers/IJCRT2008374.pdf (Isolation of nicotine dipicrate from tobacco)

 

Academic Year: