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Admissions

It is a University which is committed to excellence and overall development of the student and one where talent is nurtured and honed in the best possible manner. Come, be a part of our never-ending journey of accomplishments and accolades.

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Placements

Excellent education not only imparts knowledge, but also paves the way for a promising and successful career. The Placement Cell at The IIS University has numerous success stories to narrate in this regard...

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Life@IIS Univ

Life @ IISU is nothing less than a kaleidoscope of myriad hues; complete with vibrant events and memorable occasions to cherish for a lifetime.

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Academics

Taking the age-old tradition of enlightenment through education forward, The IIS University - in its continuous pursuit of excellence - firmly believes that an institution’s academic profile determines its worth and value. Therefore, on offer are a wide variety of both conventional and contemporary programmes of study.

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Research

To stimulate curiosity and cater to the global needs of a dynamic education, The IIS University - since inception - has been promoting and encouraging research-based experiential learning among all its students.

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About Us

The IIS University, with its perfect amalgamation of innovation, inspiration and synergy, aims to be a model institution for students across disciplines and programmes.

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IIS Univ
  • Admissions

    It is a University which is committed to excellence and overall development of the student...

  • Placements

    Excellent education not only imparts knowledge, but also paves the way for...

  • Life@IIS Univ

    Life @ IISU is nothing less than a kaleidoscope of myriad hues; complete with...

  • Academics

    Taking the age-old tradition of enlightenment through education forward,...

  • Research

    To stimulate curiosity and cater to the global needs of a dynamic education,...

  • About Us

    The IIS University, with its perfect amalgamation of innovation, inspiration...

IIS (Deemed to be University)

Gurukul Marg, SFS, Mansarovar, Jaipur 302020, (Raj.) India

Phone:- +91-141-2400160-61, 2397906-07,

Fax: 2395494,2781158, Toll Free No.: 1800 180 7750

Analytical Biochemistry | Chemistry

Analytical Biochemistry

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Paper Code: 
BCH 202
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
9.00
Unit I: 
Chromatography-I

General principles and applications of:

  1. Adsorption Chromatography
  2. Ion-exchange Chromatography
  3. Thin-layer Chromatography
  4. Molecular-sieve Chromatography
  5. Hydrophobic Chromatography
     
9.00
Unit II: 
Chromatography-II

General principles and applications of:

  1. Gas-liquid chromatography
  2. HPLC
  3. Affinity chromatography
  4. Paper chromatography
     
9.00
Unit III: 
Electrophoretic Techniques

General principles. Factors affecting the migration rate- simple, electric field, buffer and supporting medium. Basic principles of agarose electrophoresis, PAGE and SDS-PAGE, Two-dimensional electrophoresis, its importance. Isoelectrofocussing.

9.00
Unit IV: 
Immunological Techniques

Immunoassays for infectious disease- Innate immunity, antigen-specific immunity and antibodies, cell-mediated immunity and Immune memory, antibody titers, skin tests and the diagnosis of infectious disease.
Radioimmunoassay and immunoblot procedures, immunodiffusion,  immunoelectrophoresis, ELISA, immunofluorescence.
 

9.00
Unit V: 
Radioisotopic Techniques

Types of radioactive decay-half life. Types of radioisotopes used in Biochemistry, units of radioactivity measurements, techniques used to measure radioactivity (gas ionization and liquid scintillation counting), nuclear emulsions used in biological studies (pre-mounted, liquid and stripping), isotopes commonly used in biochemical studies - 32P, 35S, 14C and 3H), Autoradiography. Biological hazards of radiation and safety measures in handling radioisotopes.
 

References: 
  1. Outlines of Biochemistry, Fifth edition, Eric E. Conn, Paul K. Stumpf, George Bruening, Roy H.   Doi, John Wiley & Sons, New Delhi, 2007.
  2. Physical Biochemistry, Second edition, David Friefelder, W.H. Freeman and Co., USA, 1983.
  3. Vogel’s Textbook of Quantitative Chemical Analysis, Fifth Edition, G.H. Jeffery, J. Bassett. J. Mendham, R.C. Denney, ELBS, England, 1991.
  4. Vogel’s Text Book of Practical Organic Chemistry, Fifth Edition, B.S. Furniss, A.J. Hannaford, P.W.G. Smith, A.R. Tatchell; Adission – Wesley Longman Ltd. England, 1998.
  5. An Introduction to Practical Biochemistry, Third edition, Plummer, Tata Mc Graw Hill, 2002.
  6. Brock Biology of Microorganisms, International edition, Ninth edition, Michael T. Madigan, John M. Martinko, Pearson Prentice Hall, 2000.
     
Academic Year: 
2018-2019
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