Analytical Chemistry

Paper Code: 
CHY 414
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

Course Objectives:

This course will enable the students to -

  1. make the students understand the concept of basic analytical techniques.
  2. discuss optical methods of analysis and chromatographic separation techniques.

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

 

 

 

 

 

CHY-414

Analytical Chemistry

 

 

 

 

 

The students will be able to –

 

CO113: understand basic concepts and applications of Potentiometry, Electrogravimetry and Coulometry.

CO114: explain basic concepts and know the applications of Polarography, Polarimetry and Solvent Extraction methods.

CO115: illustrate various optical methods of analysis such as Spectrophotocolorimetry, Atomic absorption spectroscopy and Flame Photometry

CO116: describe and classify various chromatographic methods.

CO117: understand the principle and applications of various Chromatographic separation techniques like gas, liquid and ion exchange chromatography.

Interactive Lectures

 

Discussions

 

Tutorials

 

Problem solving

 

Presentations by Individual Student/ Group of Three Students

 

Class Tests at Periodic Intervals.

 

Written assignment(s)

 

Semester End Examination

 

 

 

9.00
Unit I: 
Basic Analytical Techniques I

Potentiometry– Basic concepts and Applications in Acid-base titrations, oxidation-reduction titrations, precipitation titrations and complexometric titrations.

Electrogravimetry-Basic concept: Current density, polarization, decomposition voltage, electrolysis at constant current and constant voltage and its basic applications.

Coulometry- Basic concepts: Constant current coulometry, constant potential coulometry, applications in neutralization titration, precipitation titration, oxidation- reduction titration.

 

 

9.00
Unit II: 
Basic Analytical Techniques II

Polarography – introduction, polarographic measurement, half wave potential, residual current, migration current, diffusion current, Ilkovic equation, polarographic cell and dropping mercury electrode. Precautions and advantages, applications in quantitative and qualitative analysis.

Polarimetry- polarization of light, optical activity, angle of rotation, specific rotation, measurement of rotatory power (instrumentation), application of polarimetry in quantitative and qualitative analysis.

Solvent extraction- distribution law, process of extraction (liquid- liquid, solid-liquid), techniques for solvent extraction, classification and applications.

9.00
Unit III: 
Optical Methods of Analysis

Pre requisite: Lambert and Beer law, verification, derivation, signification of λmax and molar absorptivity, theory of fluorescence and phosphorescence.

 

Spectrophotocolorimetry: Single beam and double beam spectrophotometers, functions of the components, applications.

Atomic absorption spectroscopy: PPrinciple, instrumentation, spectral interferences and chemical interferences in atomic absorption spectroscopy, applications in quantitative analysis (analysis of Zn2+, Cu2+ and Pb2+).

Flame photometry: Principle, instrumentation, interferences in flame photometry, applications in quantitative analysis. Comparison of atomic absorption and flame emission spectroscopy.

 

 

 

9.00
Unit IV: 
Chromatographic Separations I

General description and classification of chromatographic methods, thin layer, paper and column chromatographic techniques and their simple applications, Rf-values and their significance, elution in column chromatography, migration rates of solutes, band broadening and column efficiency, column resolution.

9.00
Unit V: 
Chromatographic Separations II

Principle, instrumentation, and applications of gas chromatography, high performance liquid chromatography and ion exchange chromatography.

References: 
  • Instrumental Methods of Analysis; Seventh Edition;Willard, Hobert H. et. al, CBS, 2004.
  • Analytical Chemistry, Seventh Edition,Christian, Gary D; John Willy New York, 2013.
  • Exploring Chemical Analysis, Fifth Edition;Harris, Daniel C; W.H.Freeman, New York, 2012.
  • Basic Concepts of Analytical Chemistry; Fourth Edition;Khopkar, S.M.,New AgeInternationalPvt Ltd, 2020.
  • Principles of Instrumental Analysis;Seventh Edition; Skoog, D.A., Holler F.J. and S.R. Crouch; Cengage Learning, USA, 2016.
  • Laboratory Hand Book of Chromatographic & Allied Methods; Mikes, O. &Chalmes, R.A;Halsted Press; 1979.
  • Analytical Chemistry-Methods of Separation; Ditts, R.V.; Van Nostrand, 1974.
 
Academic Year: 
2020-2021
  • Printer-friendly version
  • PDF version