CHEMISTRY LABORATORY III

Paper Code: 
C-CHY 213
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objective(s):
This course will enable the students to-

explain the core concepts of purification through crystallization and to inspect the purity through melting point and boiling point along with thin layer & paper chromatography.

 

Course Outcomes (COs):

Course Outcomes

Teaching Learning

Strategies

Assessment

Strategies

On completion of this course, the students will be able to-

CO28: understand the importance of purification in organic chemistry.

CO29: apply the knowledge to determine the melting and boiling points and judge the extent of purity present in the compound.

CO30: apply the concepts of ascending and horizontal paper chromatography for separation and to check the purity of organic compounds.

CO31: illustrate the concept of thin layer chromatography (TLC) for separation of compounds and analyse their purity.

CO32: develop their own solvent systems to separate a mixture of compounds.
  • Laboratory

instructions and demonstrations

 
  • Conduction of experiments
  • Lab records
  • Viva voce

 

 

60.00
Unit I: 
  • Checking the calibration of the thermometer 
  • Purification of organic compounds by crystallization using the following solvents: a. Water b. Alcohol c. Alcohol-Water                                    
  • Determination of the melting points of above compounds and unknown organic compounds (electrically heated melting point apparatus)                   
  • Effect of impurities on the melting point: mixed melting point of two unknown organic compounds
  • Determination of boiling point of liquid compounds. (boiling point lower than and more than 100 °C by distillation and capillary method)
     

Chromatography

Theory: Classification, principle and working of Thin Layer and Paper chromatography.

Experiments:                                                                                                    

  • Separation of a mixture of two amino acids by ascending and horizontal paper chromatography.
  • Separation of a mixture of two sugars by ascending paper chromatography.
  • Separation of a mixture of o-and p-nitrophenol or o-and p-aminophenol by thin layer chromatography (TLC)

 

 

Essential Readings: 
  • Practical Organic Chemistry, Mann, F.G. & Saunders, B.C., Pearson Education, 2009

References: 

SUGGESTED READINGS:

  • Advanced Practical Organic Chemistry, Jag Mohan, Himalaya Publishing House ,1992.
  • Vogel’s Textbook of Practical Organic Chemistry, Fifth Edition; B.S. Furniss, A.J. Hannaford, P.W.D. Smith, A.R. Tatchell; Pearson Education, New Delhi, 2003.

e-RESOURCES:

 

Scheme of Examination

The duration of examination will be 5 hrs.
The following exercises will be set in the examination
Crystallization of given compound                15 marks
Determination of b.p./m.p.                         10 marks
Separation of organic compound
using chromatographic technique                30 marks
Viva                                                         15 marks
Total                                                          70 marks

Viva

Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understood the basic principles.

 

Academic Year: 
2023-2024
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