Coming soon
Chemistry Laboratory III
This course will enable the students to
synthesize some organic compounds, purify them through crystallization and to inspect the purity through melting point and boiling point along with thin layer & paper chromatography.
Course outcomes (COs):
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Course |
Learning Outcome (at course level) |
Learning and Teaching Strategies |
Assessment Strategies |
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Course Code |
Course title |
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24CCHY 213
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Chemistry Laboratory III (Practical)
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CO36: Synthesize organic compounds and, purify them using various solvents, including water, alcohol, and alcohol-water mixtures for crystallization. CO37: Determine the melting points of organic compounds, and evaluate the effect of impurities on melting point. CO38: Apply distillation and capillary methods to determine the boiling points of liquid compounds. CO39: Separate the components of a given mixture using paper and thin layer chromatography (TLC). CO40: Compile and report the results efficiently, and develop communications skills. CO41: Contribute effectively in course-specific interaction. |
Approach in teaching: Power point presentation, laboratory instructions and demonstrations.
Learning activities for the students: Peer learning, hands-on-learning, interpretation of data, record writing and learning through virtual labs.
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Conduction of experiments, lab records, viva voce.
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Organic Synthesis
- Acetylation of aniline using green approach
- Bromination of acetanilide by conventional method/green approach
- Nitration of acetanilide by conventional method
- S-Benzylisothiouronium salt of of benzoic acid and oxalic acid.
Purification of organic compounds
- Crystallization of synthesized organic compounds using the following solvents: a. Water b. Alcohol c. Alcohol-Water
- Determination of the melting points of above compounds and unknown organic compounds (electrically heated melting point apparatus)
- Effect of impurities on the melting point: mixed melting point of two unknown organic compound.
- Determination of boiling point of liquid compounds. (boiling point lower than and more than 100 °C by distillation and capillary method)
Chromatography
Theory: Classification, principle and working of Thin Layer and Paper chromatography.
Experiments:
- Separation of a mixture of two amino acids by ascending and horizontal paper chromatography.
- Separation of a mixture of two sugars by ascending paper chromatography.
- Separation of a mixture of o-and p-nitrophenol or o-and p-aminophenol by thin layer chromatography (TLC)
Scheme of Examination
The duration of examination will be 5 hrs.
The following exercises will be set in the examination
Synthesis and crystallization of organic compound 30 marks
Determination of b.p./m.p. 10 marks
Separation of organic compound using chromatographic technique 15 marks
Viva 15 marks
Total 70 marks
Viva
Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understood the basic principles.
- Practical Organic Chemistry, Mann, F.G. & Saunders, B.C., Pearson Education, 2009
- Advanced Practical Organic Chemistry, Jag Mohan, Himalaya Publishing House ,1992.
- Vogel’s Textbook of Practical Organic Chemistry, Fifth Edition; B.S. Furniss, A.J. Hannaford, P.W.D. Smith, A.R. Tatchell; Pearson Education, New Delhi, 2003.
e-Resources:
- https://www.slideshare.net/LavakusaBanavatu/thin-layer-chromatography-43293607 (TLC)
- https://www.studocu.com/in/u/23692705?sid=01684403081 (all the experiments)
- https://www.slideshare.net/msaltyy/paper-chromatography-65615443 (Paper Chromatography)
