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CHEMISTRY LABORATORY IX
Course Objective(s):
This course will enable the students to-
understand the handling of UV-vis spectrophotometer and its use in various applications.
Course Outcomes (COs):
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Course Outcomes |
Teaching Learning Strategies |
Assessment Strategies |
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On completion of this course, the students will be able to- CO86: determine the rate constant and order of a chemical reaction using spectrophotometer techniques. CO87: determine the pKa value of a weak acid using spectrophotometer, and relate the pKa value to the acid's strength. CO88: use spectrophotometer as analytical techniques to estimate the concentration of a given chemical species in a sample. |
instructions and demonstrations
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Minor Experiments
- Study the absorbance spectra of KMnO4 and K2Cr2O7 (in 0.1 M H2SO4) and determine the λmax values. Calculate the energies of the two transitions indifferent units (J molecule-1, kJ mol-1, cm-1, eV).
- Study the pH-dependence of the UV-Vis spectrum of K2Cr2O7.
- Record the 200-350 nm UV spectra of the given compounds (acetone, acetaldehyde, 2-propanol, acetic acid) in water. Comment on the effect of structure on the UV spectra of organic compounds.
- Analyse the given vibrational-rotational spectrum of HCl.
Major Experiments
- Verify Lambert-Beer’s law and determine the concentration of CuSO4/KMnO4/K2Cr2O7 in a solution of unknown concentration.
- Determine the concentrations of KMnO4 and K2Cr2O7 in a mixture spectrophotometrically.
- Study the kinetics of iodination of propanone in acidic medium.(volumetrically/spectrophotometrically)
- Determine the amount of iron present in a sample using 1,10-phenathroline spectrophotometrically.
- Determine the dissociation constant of an indicator phenolphthalein spectrophotometrically.
- Study the kinetics of interaction of crystal violet/ phenolphthalein with sodium hydroxide. (volumetrically/spectrophotometrically)
- Advanced Practical Physical Chemistry, Thirtieth Edition; J. B. Yadav; Krishna Prakashan Media Pvt. Ltd., Meerut, 2015.
SUGGESTED READINGS:
- Experimental Physical Chemistry, First Edition; V. D. Athawale and P. Mathur; New age International (P) Ltd. Publishers, New Delhi, 2011.
- Experiments in Physical Chemistry, Fifth Edition; D. P. Shoemaker, C. W. Garland and J. W. Nibler, Mc Graw-Hill, New York, 1998.
e-RESOURCES:
- https://vlab.amrita.edu/?sub=2&brch=190&sim=338&cnt=1 (spectrophotometry)
- http://mas-iiith.vlabs.ac.in/List%20of%20experiments.html (kinetics, pH effect, verify Beer-Lambert’s law)
Scheme of Examination
The duration of examination will be 5 hrs.
The following exercises will be set in the examination
Minor experiment 20 marks
Major experiment 35 marks
Viva 15 marks
Total 70 marks
The candidates shall be given two exercise out of exercises prescribed in the syllabus. The exercises in physical chemistry can be modified by the examiner and the same will be explained to the candidates. However, such modifications will not involve any departure from the basic principles over which experiments are prescribed in the syllabus.
The distribution of marks will be as follows
Minor Major
Theory and procedure 2 marks 5 marks
Correct observation 6 marks 12 marks
Use of correct formula 2 marks 5 marks
Calculation/Interpretation 6 marks 8 marks
Results 4 marks 5 marks
Viva
Oral questions shall generally be asked over the work assigned to the candidate. The purpose is to find out as to what extent the candidate has understanding the basic principles.
